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Gene Review

RHO4  -  Rho family GTPase RHO4

Saccharomyces cerevisiae S288c

Synonyms: GTP-binding protein RHO4, YKR055W
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High impact information on RHO4

  • Bnr1p interacts with another Rho family member, Rho4p, but not with Rho1p [1].
  • Overproduction of either Rho3p or the Rho3p-related GTPase Rho4p suppressed the boi defect [2].
  • We found here that a novel Src homology 3 (SH3) domain-containing protein, encoded by YMR032w, interacted with Bnr1p in a GTP-Rho4p-dependent manner through the FH1 domain of Bnr1p and the SH3 domain of Ymr032wp [3].
  • In addition, these results strongly argue in favour of Rho3p and Rho4p being the targets of Rgd1p RhoGAP activity in vivo [4].
  • We hypothesize that the rgd1Delta inactivation, at least through the hyperactivation of the small GTPases Rho3p and Rho4p, alters the secretory pathway and/or the actin cytoskeleton and decreases activity of the PKC pathway [5].

Biological context of RHO4


Physical interactions of RHO4

  • The Rho3 and Rho4 small GTPases interact functionally with Wsc1p, a cell surface sensor of the protein kinase C cell-integrity pathway in Saccharomyces cerevisiae [6].

Other interactions of RHO4

  • Analysis of the synthetic lethal phenomenon revealed that production of activated Rho3p and Rho4p leads to lethality in wsc1Delta cells [6].


  1. Bni1p and Bnr1p: downstream targets of the Rho family small G-proteins which interact with profilin and regulate actin cytoskeleton in Saccharomyces cerevisiae. Imamura, H., Tanaka, K., Hihara, T., Umikawa, M., Kamei, T., Takahashi, K., Sasaki, T., Takai, Y. EMBO J. (1997) [Pubmed]
  2. Yeast src homology region 3 domain-binding proteins involved in bud formation. Matsui, Y., Matsui, R., Akada, R., Toh-e, A. J. Cell Biol. (1996) [Pubmed]
  3. Interaction of Bnr1p with a novel Src homology 3 domain-containing Hof1p. Implication in cytokinesis in Saccharomyces cerevisiae. Kamei, T., Tanaka, K., Hihara, T., Umikawa, M., Imamura, H., Kikyo, M., Ozaki, K., Takai, Y. J. Biol. Chem. (1998) [Pubmed]
  4. Evidence for the genetic interaction between the actin-binding protein Vrp1 and the RhoGAP Rgd1 mediated through Rho3p and Rho4p in Saccharomyces cerevisiae. Roumanie, O., Peypouquet, M.F., Bonneu, M., Thoraval, D., Doignon, F., Crouzet, M. Mol. Microbiol. (2000) [Pubmed]
  5. Overactivation of the protein kinase C-signaling pathway suppresses the defects of cells lacking the Rho3/Rho4-GAP Rgd1p in Saccharomyces cerevisiae. de Bettignies, G., Thoraval, D., Morel, C., Peypouquet, M.F., Crouzet, M. Genetics (2001) [Pubmed]
  6. The Rho3 and Rho4 small GTPases interact functionally with Wsc1p, a cell surface sensor of the protein kinase C cell-integrity pathway in Saccharomyces cerevisiae. Fernandes, H., Roumanie, O., Claret, S., Gatti, X., Thoraval, D., Doignon, F., Crouzet, M. Microbiology (Reading, Engl.) (2006) [Pubmed]
  7. PCR-based gene targeting in the filamentous fungus Ashbya gossypii. Wendland, J., Ayad-Durieux, Y., Knechtle, P., Rebischung, C., Philippsen, P. Gene (2000) [Pubmed]
  8. Isolation and characterization of two novel ras superfamily genes in Saccharomyces cerevisiae. Matsui, Y., Toh-e, A. Gene (1992) [Pubmed]
  9. RGD1, encoding a RhoGAP involved in low-pH survival, is an Msn2p/Msn4p regulated gene in Saccharomyces cerevisiae. Gatti, X., de Bettignies, G., Claret, S., Doignon, F., Crouzet, M., Thoraval, D. Gene (2005) [Pubmed]
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