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Gene Review

ARF3  -  Arf family GTPase ARF3

Saccharomyces cerevisiae S288c

Synonyms: ADP-ribosylation factor 3, YOR094W, YOR3172W
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Disease relevance of ARF3


High impact information on ARF3

  • A 200-kDa guanine nucleotide-exchange protein (p200 or GEP) for ADP-ribosylation factors 1 and 3 (ARF1 and ARF3) that was inhibited by brefeldin A (BFA) was purified earlier from cytosol of bovine brain cortex [2].
  • Role for Arf3p in development of polarity, but not endocytosis, in Saccharomyces cerevisiae [3].
  • The nucleotide-binding, N-terminal region, and N-terminal myristate of Arf3p are important for its proper localization [3].
  • Yeast Arf3p, which is most similar to mammalian ARF6, is not essential for cell viability and not required for endoplasmic reticulum-to-Golgi protein transport [3].
  • Both haploid and homologous diploid arf3 mutants exhibit a random budding defect, and the overexpression of the GTP-bound form Arf3p(Q71L) or GDP-binding defective Arf3p(T31N) mutant interfered with budding-site selection [3].

Biological context of ARF3


Anatomical context of ARF3

  • Taken together, our data provide further evidence for the role of Lsb5p in membrane-trafficking events at the plasma membrane and also demonstrate for the first time an interaction of Arf3 with the endocytic machinery in yeast [5].

Other interactions of ARF3

  • Our findings demonstrate that Afi1p functions as an Arf3p polarization-specific adapter and participates in development of polarity [6].


  1. Reverse two-hybrid techniques in the yeast Saccharomyces cerevisiae. Bennett, M.A., Shern, J.F., Kahn, R.A. Methods Mol. Biol. (2004) [Pubmed]
  2. Cloning and expression of a cDNA encoding a bovine brain brefeldin A-sensitive guanine nucleotide-exchange protein for ADP-ribosylation factor. Morinaga, N., Moss, J., Vaughan, M. Proc. Natl. Acad. Sci. U.S.A. (1997) [Pubmed]
  3. Role for Arf3p in development of polarity, but not endocytosis, in Saccharomyces cerevisiae. Huang, C.F., Liu, Y.W., Tung, L., Lin, C.H., Lee, F.J. Mol. Biol. Cell (2003) [Pubmed]
  4. A family of ADP-ribosylation factor effectors that can alter membrane transport through the trans-Golgi. Boman, A.L., Zhang, C., Zhu, X., Kahn, R.A. Mol. Biol. Cell (2000) [Pubmed]
  5. Lsb5p interacts with actin regulators Sla1p and Las17p, ubiquitin and Arf3p to couple actin dynamics to membrane trafficking processes. Costa, R., Warren, D.T., Ayscough, K.R. Biochem. J. (2005) [Pubmed]
  6. Afi1p functions as an Arf3p polarization-specific docking factor for development of polarity. Tsai, P.C., Lee, S.W., Liu, Y.W., Chu, C.W., Chen, K.Y., Ho, J.C., Lee, F.J. J. Biol. Chem. (2008) [Pubmed]
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