The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
Gene Review

LAS17  -  Las17p

Saccharomyces cerevisiae S288c

Synonyms: Proline-rich protein LAS17, YOR181W
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of LAS17


High impact information on LAS17

  • Catalytically inactive Lsb6 interacted with Las17 and promoted endosome motility [2].
  • We showed previously that the yeast orthologue of the Wiskott-Aldrich Syndrome protein, Bee1/Las17p, and the type I myosins are key regulators of cortical actin polymerization [3].
  • Its interactions with Las17p, Vrp1p, and the type I myosins are essential for this process [1].
  • Here, we report the characterization of Lsb7/Bzz1p (for Las seventeen binding protein 7), an Src homology 3 (SH3) domain protein that interacts directly with Las17p via a polyproline-SH3 interaction [1].
  • The exocytic SNARE Snc1p, which is recycled by an endocytic route, was also intracellularly accumulated, and inhibition of endocytic internalization suppressed the cytoplasmic accumulation of both Las17p and Snc1p [4].

Biological context of LAS17

  • The actin patch assembly mutants las17 delta, sla1delta and vrp1 delta showed elevated levels of cell wall beta-1,6-glucan, and mutant cells were capable of only a limited number of cell divisions compared to wild-type [5].
  • Interestingly, five ORFs were preys of both Rvs161p and Rvs167p (RVS167, LAS17, YNL094w, YMR192w and YPL249c) [6].
  • Moreover, a high cell survival rate was attained with overexpression of LAS17, when cells in the stationary phase of the growth cycle were subjected to heat killing (48 degrees C) or ethanol killing (20% v/v) [7].
  • Sake yeast strain UT-1 grows at a faster rate as a result of the overexpression of LAS17 than control cultures under various stresses such as high temperature, high ethanol concentration, and oxidative stress, and the tolerance to these stresses was increased compared with the control [7].
  • The effects of the overexpression of LAS17/BEE1, which encodes a yeast protein exhibiting sequence homology to the Wiscott-Aldrich syndrome protein, on the cell growth of Saccharomyces cerevisiae were examined [7].

Associations of LAS17 with chemical compounds


Regulatory relationships of LAS17

  • The addition of Las17p WA further enhances yeast actin polymerization by yArp2/3 and allows the complex to now assist muscle actin polymerization [8].

Other interactions of LAS17

  • It colocalizes with cortical actin patches and with Las17p [1].
  • In the present study, we have further defined its interaction site with both Sla1p and with Las17p, two regulators of actin dynamics [9].
  • Las17 was a much stronger activator of Arp2/3 complex than its carboxyl-terminal (WA) fragment [10].

Analytical, diagnostic and therapeutic context of LAS17


  1. Saccharomyces cerevisiae Bzz1p is implicated with type I myosins in actin patch polarization and is able to recruit actin-polymerizing machinery in vitro. Soulard, A., Lechler, T., Spiridonov, V., Shevchenko, A., Shevchenko, A., Li, R., Winsor, B. Mol. Cell. Biol. (2002) [Pubmed]
  2. A WASp-binding type II phosphatidylinositol 4-kinase required for actin polymerization-driven endosome motility. Chang, F.S., Han, G.S., Carman, G.M., Blumer, K.J. J. Cell Biol. (2005) [Pubmed]
  3. A two-tiered mechanism by which Cdc42 controls the localization and activation of an Arp2/3-activating motor complex in yeast. Lechler, T., Jonsdottir, G.A., Klee, S.K., Pellman, D., Li, R. J. Cell Biol. (2001) [Pubmed]
  4. Defects in structural integrity of ergosterol and the Cdc50p-Drs2p putative phospholipid translocase cause accumulation of endocytic membranes, onto which actin patches are assembled in yeast. Kishimoto, T., Yamamoto, T., Tanaka, K. Mol. Biol. Cell (2005) [Pubmed]
  5. Actin patch assembly proteins Las17p and Sla1p restrict cell wall growth to daughter cells and interact with cis-Golgi protein Kre6p. Li, H., Pagé, N., Bussey, H. Yeast (2002) [Pubmed]
  6. A network of proteins around Rvs167p and Rvs161p, two proteins related to the yeast actin cytoskeleton. Bon, E., Recordon-Navarro, P., Durrens, P., Iwase, M., Toh-E, A., Aigle, M. Yeast (2000) [Pubmed]
  7. Effect of overexpression of LAS17 on stress tolerance and the stability of extrachromosomal DNA in Saccharomyces cerevisiae. Mizoguchi, H., Hara, S. J. Biosci. Bioeng. (2001) [Pubmed]
  8. Acceleration of yeast actin polymerization by yeast Arp2/3 complex does not require an Arp2/3-activating protein. Wen, K.K., Rubenstein, P.A. J. Biol. Chem. (2005) [Pubmed]
  9. Lsb5p interacts with actin regulators Sla1p and Las17p, ubiquitin and Arf3p to couple actin dynamics to membrane trafficking processes. Costa, R., Warren, D.T., Ayscough, K.R. Biochem. J. (2005) [Pubmed]
  10. Negative regulation of yeast WASp by two SH3 domain-containing proteins. Rodal, A.A., Manning, A.L., Goode, B.L., Drubin, D.G. Curr. Biol. (2003) [Pubmed]
WikiGenes - Universities