The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)



Gene Review

TBF1  -  Tbf1p

Saccharomyces cerevisiae S288c

Synonyms: LPI16C, Protein TBF1, TBF-alpha, TTAGGG repeat-binding factor 1, YPL128C
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

High impact information on TBF1

  • Because poly(C.A)/poly(T.G) sequences from a wide range of organisms will serve as the primer for the in vivo extension of telomeres in yeast, TBF alpha is of particular interest [1].
  • A possible function for TBF alpha might be to serve as an anchoring protein for the yeast telomerase by binding to the conserved junction sequence at a distance from the terminus to allow addition of an irregular repeating sequence at the chromosome end [1].
  • The Tbf1 and Reb1 proteins are present in yeast subtelomeric regions [2].
  • The DNA sequence of TBF1 was determined [3].
  • Diploid cells heterozygous for a tbf1::URA3 insertion mutation are viable but upon sporulation give rise to tetrads with only two viable spores, both of which are Ura-, indicating that the TBF1 gene is essential for growth [3].

Biological context of TBF1

  • The yeast TBF1 gene is essential for mitotic growth and encodes a protein that binds the human telomere repeats in vitro, although its cellular function is unknown [4].
  • We propose to call the particular Myb-related motif found in these proteins the 'telobox'. Antibodies directed against the Tbf1 telobox detect two proteins in nuclear and mitotic chromosome extracts from human cell lines [5].
  • Identification of high affinity Tbf1p-binding sites within the budding yeast genome [4].
  • PCR-assisted binding site selection was used to define a consensus for high affinity Tbf1p-binding sites [4].
  • Immunofluorescence of Tbf1p shows a spotty pattern throughout the interphase nucleus and along synapsed chromosomes in meiosis, suggesting that Tbf1p binds internal chromosomal sites in addition to sub-telomeric regions [4].

Associations of TBF1 with chemical compounds

  • TBF1 produced in vitro yields protein-DNA complexes with (TTAGGG)n probes that have mobilities on native polyacrylamide gels identical to those produced by partially purified TBF alpha from yeast cells [3].

Physical interactions of TBF1

  • In this study we show that the Tbf1p DNA-binding domain can target the Gal4 transactivation domain to a (TTAGGG)(n) sequence inserted in the yeast genome, supporting the model that Tbf1p binds this sub-telomeric repeat motif in vivo [4].

Other interactions of TBF1

  • Together with Reb1p and Tbf1p, previously involved in the activity of Saccharomyces cerevisiae subtelomeric insulators, insulating potential emerges as a supplementary common property of General Regulatory Factors (GRFs) [6].


  1. A yeast protein that binds to vertebrate telomeres and conserved yeast telomeric junctions. Liu, Z.P., Tye, B.K. Genes Dev. (1991) [Pubmed]
  2. Subtelomeric proteins negatively regulate telomere elongation in budding yeast. Berthiau, A.S., Yankulov, K., Bah, A., Revardel, E., Luciano, P., Wellinger, R.J., Géli, V., Gilson, E. EMBO J. (2006) [Pubmed]
  3. An essential yeast gene encoding a TTAGGG repeat-binding protein. Brigati, C., Kurtz, S., Balderes, D., Vidali, G., Shore, D. Mol. Cell. Biol. (1993) [Pubmed]
  4. Identification of high affinity Tbf1p-binding sites within the budding yeast genome. Koering, C.E., Fourel, G., Binet-Brasselet, E., Laroche, T., Klein, F., Gilson, E. Nucleic Acids Res. (2000) [Pubmed]
  5. The telobox, a Myb-related telomeric DNA binding motif found in proteins from yeast, plants and human. Bilaud, T., Koering, C.E., Binet-Brasselet, E., Ancelin, K., Pollice, A., Gasser, S.M., Gilson, E. Nucleic Acids Res. (1996) [Pubmed]
  6. General regulatory factors (GRFs) as genome partitioners. Fourel, G., Miyake, T., Defossez, P.A., Li, R., Gilson, E. J. Biol. Chem. (2002) [Pubmed]
WikiGenes - Universities