High impact information on REC104

• Spatial organization and dynamics of the association of Rec102 and Rec104 with meiotic chromosomes [1].
• The interactions suggest that Spo11p, Rec102p, and Rec104p may form a trimeric complex during the initiation of recombination [2].
• REC104 is a gene required for the initiation of meiotic recombination in Saccharomyces cerevisiae [3].
• To better understand the role of REC104 in meiosis, we used an in vitro mutagenesis technique to create a set of temperature-conditional mutations in REC104 and used one ts allele (rec104-8) in a screen for high-copy suppressors [3].
• Finally, if a DSB is provided by the HO endonuclease, recombination can occur in the absence of MEI4 and REC104 [4].

Biological context of REC104

• We find that mutations in any of three Early Exchange genes (REC104, REC114 or REC102) confer a phenotype in which the reductional division occurs earlier than in an isogenic wild-type diploid [5].
• The second suppressor maps to the right arm of chromosome VIII distal to CDC12 and is REC104, a meiosis-specific gene believed to act early in meiosis [6].
• Overexpression of REC104 from a 2 mu plasmid was shown to enhance the spore viability of every allele tested, including a hop1 disruption allele [7].
• The REC104 gene has no apparent role in mitosis, since mutations have no observable effect on growth, mitotic recombination, or DNA repair [8].
• The DNA sequence of REC104 reveals that it is a previously unknown gene with a coding region of 549-bp, and genetic mapping has localized the gene to chromosome VIII near FUR1 [8].

Regulatory relationships of REC104

• An increased dosage of the early exchange gene REC102 was found to suppress the conditional recombinational reduction in rec104-8 as well as in several other conditional rec104 alleles [3].

Other interactions of REC104

• However, no suppression was observed for a null allele of REC104, indicating that the suppression by REC102 is not "bypass" suppression [3].
• We demonstrate that a mutation in a gene (REC104) required for initiation of exchange is completely epistatic to a mutation in RED1 [9].
• The rec104 mutation is epistatic to the mei4 mutation for the timing of the first division [5].
• The REC104 gene was initially defined by mutations that rescued the inviability of a rad52 spo 13 haploid strain in meiosis [8].

References