High impact information on HIS1

• The gene is located about 8 kilobase pairs from HIS1 and is present as a single copy in the yeast genome [1].
• The HIS1 gene of Saccharomyces cerevisiae encodes ATP phosphoribosyltransferase, the first enzyme in the pathway of histidine biosynthesis [2].
• We determined the DNA sequence of the HIS1 region defined by this complementation test [2].
• The FCY2 gene was mapped on the right arm of chromosome V close to the HIS1 gene [3].
• The his1 gene (chromosome V) of Saccharomyces cerevisiae specifies phosphoribosyl transferase (E.C.2.4.2.17), the first enzyme of histidine biosynthesis [4].

Biological context of HIS1

• In the DNA sequence upstream from the 5' termini of HIS1 mRNA we have found four closely related copies of a 9-base pair sequence [2].
• The PRO2 gene mapped on chromosome XV tightly linked to cdc66, and the PRO3 gene was located on the right arm of chromosome V between HIS1 and the centromere [5].
• Homologous sequence are observed in HIS1, HIS3, and in TRP5 upstream regions between copies of the repeat [6].
• Cloning of the Candida albicans HIS1 gene by direct complementation of a C. albicans histidine auxotroph using an improved double-ARS shuttle vector [7].
• To verify the diploidy of the selected chromosome V, the HIS1 gene was deleted with a standard KanMX4 knockout DNA cassette [8].

Anatomical context of HIS1

• To investigate the possibility of inducing specific chromosome loss by centromere deletion in eukaryotic cells, the yeast diploid strain ZG1, carrying three pairs of heterozygous marker genes (CAN1(S)/can1(R), URA3/Deltaura3, hphMX4/HIS1), widely spread on both arms of chromosome V, was constructed [8].

Other interactions of HIS1

• Each of the his1-his7 mutant strains were unable to grow in the presence of elevated levels of Cu, Co, or Ni at nearly neutral pHs, in contrast to His(+) strains, which grew under these conditions [9].
• Treatment of S. pombe cells with 3-AT leads to a small increase in the level of the his5 transcript, but no effect is seen on the level of the his1 transcript [10].
• All 8 induced 2-5-fold increases in reversion frequencies over background at the trp5 locus--5 of these induced 1.5-3-fold increases at the hom3 locus and 1 induced a doubling at his1 [11].

Analytical, diagnostic and therapeutic context of HIS1

• The resulting heterogeneity of the HIS1/KanMX4 markers, together with quantitative PCR and densitometric analysis on chromosome V, confirmed its diploid complement, thereby indicating that an endoreduplication event had taken place [8].

References