The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
Gene Review

Rep  -  replication associated protein

Ageratum enation virus

 
 
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.
 

Disease relevance of Rep

  • Identification of Rep as resembling the family of ribonucleoprotein/RNA-recognition motif fold proteins establishes a structure-based evolutionary link between RNA binding proteins, splicing factors, and replication initiators of prokaryotic and eukaryotic single-stranded DNA elements and mammalian DNA tumor viruses [1].
  • The origin of replication of African cassava mosaic virus (ACMV) and a gene expression vector based on Potato virus X were exploited to devise an in planta system for functional analysis of the geminivirus replication-associated protein (Rep) in transgenic Nicotiana benthamiana line pOri-2 [2].
  • These results prompt a reassessment of the current model of geminivirus replication where Rep/DNA interaction is a highly specific step in the initiation of rolling circle replication [3].
 

High impact information on Rep

  • Here we present the solution NMR structure of the catalytic domain of Rep, the initiator protein of tomato yellow leaf curl virus [1].
  • The release of episomal trans replicons from the transgene and the increase in GFP expression were dependent on the cognate geminiviral replication-associated protein (Rep) and required interaction between Rep and the intergenic region of TYLCSV [4].
  • This line contains an integrated copy of a tandem repeat of the ACMV origin of replication flanking nonviral sequences that can be mobilized and replicated by Rep as an episomal replicon [2].
  • We have used a combination of protein-binding assays and mutagenesis to show that repeat motifs in TLCV and sat-DNA are essential for Rep-binding in vitro [3].
  • The DNA binding sites for the replication-associated protein (Rep) of two strains of tomato leaf curl virus from New Delhi (ToLCV-Nde) were identified using electrophoretic mobility shift assays (EMSAs) [5].
 

Biological context of Rep

  • A Rep-GFP fusion protein can also mobilize and amplify the replicon, facilitating Rep detection in planta [2].

References

  1. The structure of a replication initiator unites diverse aspects of nucleic acid metabolism. Campos-Olivas, R., Louis, J.M., Clerot, D., Gronenborn, B., Gronenborn, A.M. Proc. Natl. Acad. Sci. U.S.A. (2002) [Pubmed]
  2. Novel system for the simultaneous analysis of geminivirus DNA replication and plant interactions in Nicotiana benthamiana. Hong, Y., Stanley, J., van Wezel, R. J. Virol. (2003) [Pubmed]
  3. High-affinity Rep-binding is not required for the replication of a geminivirus DNA and its satellite. Lin, B., Akbar Behjatnia, S.A., Dry, I.B., Randles, J.W., Rezaian, M.A. Virology (2003) [Pubmed]
  4. A versatile transreplication-based system to identify cellular proteins involved in geminivirus replication. Morilla, G., Castillo, A.G., Preiss, W., Jeske, H., Bejarano, E.R. J. Virol. (2006) [Pubmed]
  5. Sequence parameters that determine specificity of binding of the replication-associated protein to its cognate site in two strains of tomato leaf curl virus-New Delhi. Chatterji, A., Chatterji, U., Beachy, R.N., Fauquet, C.M. Virology (2000) [Pubmed]
 
WikiGenes - Universities