The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
Gene Review

aldA  -  aldehyde dehydrogenase A, NAD-linked

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK1408, JW1412, ald
 
 
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.
 

Disease relevance of aldA

  • The region containing aldA and tagA comprises 13 kb of previously unidentified DNA and is part of a PAI that contains a regulator of virulence genes (ToxT) and a gene cluster encoding an essential colonization factor and the cholera toxin phage receptor (toxin-coregulated pilus; TCP) [1].
  • The aldA gene of Escherichia coli is under the control of at least three transcriptional regulators [2].
 

High impact information on aldA

  • Analysis of the 5'- and 3'-flanking sequences indicated that the ald gene is an operon [3].
  • The ald region was sequenced, and a single open reading frame of 479 codons specifying the subunit of the aldehyde dehydrogenase enzyme complex was identified [3].
  • Determination of the N-terminal amino acid sequence of the enzyme protein unambiguously established the identity and the start codon of the ald gene [3].
  • The aldA gene is the first example of a gene encoding a cytoplasmic protein that is under the control of ToxR, and this suggests that metabolic enzymes may constitute novel members of virulence regulons in bacteria [4].
  • Moreover, growth experiments on fucose indicated that a double ald fucO mutant accumulated inhibiting concentrations of lactaldehyde [5].
 

Biological context of aldA

 

Associations of aldA with chemical compounds

  • Mutants that grow on L-1,2-propanediol as a carbon and energy source also depend on the ald gene product for the conversion of L-lactaldehyde to L-lactate [7].
  • Mutations affecting this activity mapped to the ald locus at min 31, well apart from the fuc genes (min 60) encoding the trunk pathway for L-fucose dissimilation (as well as L-1,2-propanediol oxidoreductase) and the rha genes (min 88) encoding the trunk pathway for L-rhamnose dissimilation [7].
 

Other interactions of aldA

  • Fusions of different deletions of the aldA promoter to lacZ indicated that a Crp site proximal to the transcriptional start point (tsp) was functional in the cAMP-dependent catabolite repression of this system, whereas a distal control site was likely to operate in a cAMP-independent catabolite repression [2].

References

  1. A Vibrio cholerae pathogenicity island associated with epidemic and pandemic strains. Karaolis, D.K., Johnson, J.A., Bailey, C.C., Boedeker, E.C., Kaper, J.B., Reeves, P.R. Proc. Natl. Acad. Sci. U.S.A. (1998) [Pubmed]
  2. The aldA gene of Escherichia coli is under the control of at least three transcriptional regulators. Limón, A., Hidalgo, E., Aguilar, J. Microbiology (Reading, Engl.) (1997) [Pubmed]
  3. Molecular cloning and DNA sequencing of the Escherichia coli K-12 ald gene encoding aldehyde dehydrogenase. Hidalgo, E., Chen, Y.M., Lin, E.C., Aguilar, J. J. Bacteriol. (1991) [Pubmed]
  4. Expression of the Vibrio cholerae gene encoding aldehyde dehydrogenase is under control of ToxR, the cholera toxin transcriptional activator. Parsot, C., Mekalanos, J.J. J. Bacteriol. (1991) [Pubmed]
  5. L-1,2-propanediol exits more rapidly than L-lactaldehyde from Escherichia coli. Zhu, Y., Lin, E.C. J. Bacteriol. (1989) [Pubmed]
  6. Anomalous DnaA protein binding to the regulatory region of the Escherichia coli aldA gene. Ozaki, T., Kumaki, Y., Kitagawa, R., Ogawa, T. Microbiology (Reading, Engl.) (2001) [Pubmed]
  7. NAD-linked aldehyde dehydrogenase for aerobic utilization of L-fucose and L-rhamnose by Escherichia coli. Chen, Y.M., Zhu, Y., Lin, E.C. J. Bacteriol. (1987) [Pubmed]
 
WikiGenes - Universities