Disease relevance of eda

• Sequence of the Escherichia coli K-12 edd and eda genes of the Entner-Doudoroff pathway [1].
• The pathways of pectin and galacturonate catabolism in Erwinia chrysanthemi converge to form a common intermediate, 2-keto-3-deoxygluconate, which is phosphorylated to form 2-keto-3-deoxy-6-phosphogluconate (KDGP) and then cleaved by the aldolase encoded by the kdgA gene [2].

High impact information on eda

• The nucleotide sequence of the kdgA region indicated that the kdgA reading frame is 639 bases long, corresponding to a protein of 213 amino acids with a molecular mass of 22,187 Da [2].
• We cloned the kdgA gene of the E. chrysanthemi strain 3937 by complementing an Escherichia coli kdgA mutation, using an RP4-derivative plasmid [2].
• Despite the presence of potential KdgR binding sites (KdgR-box) in the regulatory regions of four genes involved in pectin catabolism (kdgC, kduD, pem, kdgA) and in a pectinase secretion gene outC, no DNA-repressor complex could be observed by in vitro experiments [3].
• Neither the E. coli F-18 eda nor the E. coli K-12 eda mutant was able to colonize the streptomycin-treated mouse intestine, whether they were fed to mice together with their respective parental strains or alone [4].
• Other proteins affected by the reduced GroE level included two proteins (enzymes of the Entner-Doudoroff pathway) encoded by the edd-eda operon and the ribosomal protein S6, suggesting that the GroE chaperones are involved in regulating expression of genes for carbohydrate metabolism and in modulating biogenesis or function of the ribosome [5].

Chemical compound and disease context of eda

• When E. coli is grown on gluconate, the edd and eda genes are cotranscribed [6].
• The eda gene that encodes 2-keto-3-deoxy-6-phosphogluconate aldolase of the Entner-Doudoroff pathway was cloned from Zymomonas mobilis by genetic complementation of an Escherichia coli mutant [7].

Biological context of eda

• The nucleotide sequence of the entire Escherichia coli edd-eda region that encodes the enzymes of the Entner-Doudoroff pathway was determined [6].
• The eda reading frame is 642 bases long and encodes the 213-amino-acid, 22,283-Da protein 2-keto-3-deoxy-6-phosphogluconate aldolase [6].
• Molecular characterization of the Entner-Doudoroff pathway in Escherichia coli: sequence analysis and localization of promoters for the edd-eda operon [6].

Other interactions of eda

• Locations of the zwf, edd, and eda genes on the Escherichia coli physical map [8].

Analytical, diagnostic and therapeutic context of eda

• Restriction mapping of the kdgA region and isolation of kdgA-lac fusions allowed the more precise localization of the kdgA gene and determination of its transcriptional direction [2].

References