The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
Gene Review

ackA  -  acetate kinase A and propionate kinase 2

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK2290, JW2293
 
 
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.
 

Disease relevance of ackA

 

High impact information on ackA

  • Relative to wild-type cells, those deleted for acs did not activate acetate as well, those deleted for ackA and pta displayed even less activity, and those deleted for all three genes did not activate acetate at any concentration tested [2].
  • The complete nucleotide sequence of the ackA gene was determined [3].
  • The ackA gene was found to be located about 15 kilobases upstream of the purF-folC-hisT region of the chromosome [3].
  • The mRNA levels of ackA and proU were high during the batch growth phase, but declined drastically when glucose became limited, whereas the mRNA levels of the other stress genes were relatively constant throughout the process [4].
  • Our laboratory previously studied the interaction between nuo and the acetate-producing pathway encoded by ackA-pta in Escherichia coli [5].
 

Chemical compound and disease context of ackA

  • Although the bacterium E. coli is chosen as the host in many bioprocesses, products derived from the central aerobic metabolic pathway often compete with the acetate-producing pathways poxB and ackA-pta for glucose as the substrate [6].
  • A recombinant E. coli strain with a deletion in ackA-pta produces less acetate and more isoamyl acetate than the wild-type E. coli strain [6].
 

Biological context of ackA

  • A 3,700 nucleotide transcript, which covers the ackA and pta genes, seemed to be produced by the first promoter in the operon and a 2,300 nucleotide transcript, which covers just pta, seemed to be produced by the second promoter [1].
  • Nucleotide sequencing of the pta gene revealed that it is able to produce a polypeptide comprising 714 amino acid residues, which starts at 70 base pairs downstream from the stop codon of the ackA gene [1].
  • Conditional ackA silencing reduced carbon flux to acetate and increased heterologous gene expression [7].
  • PTasRNAs targeted against the ackA gene within the acetate kinase-phosphotransacetylase operon (ackA-pta) triggered target mRNA decay and a 78% reduction in AckA activity with high genetic penetrance [7].
  • A gene sharing high similarity scores with ackA genes, encoding the acetate kinase, was found upstream from the rbs gene cluster [8].
 

Associations of ackA with chemical compounds

  • The ackA-pta mutant has a pfl::lacZ expression level much higher than that of the wild-type strain, and cultures also exhibit the highest ethanol production [5].

References

  1. Identification and characterization of the ackA (acetate kinase A)-pta (phosphotransacetylase) operon and complementation analysis of acetate utilization by an ackA-pta deletion mutant of Escherichia coli. Kakuda, H., Hosono, K., Shiroishi, K., Ichihara, S. J. Biochem. (1994) [Pubmed]
  2. Cloning, characterization, and functional expression of acs, the gene which encodes acetyl coenzyme A synthetase in Escherichia coli. Kumari, S., Tishel, R., Eisenbach, M., Wolfe, A.J. J. Bacteriol. (1995) [Pubmed]
  3. Cloning, expression, and nucleotide sequence of the Escherichia coli K-12 ackA gene. Matsuyama, A., Yamamoto, H., Nakano, E. J. Bacteriol. (1989) [Pubmed]
  4. Monitoring of genes that respond to process-related stress in large-scale bioprocesses. Schweder, T., Krüger, E., Xu, B., Jürgen, B., Blomsten, G., Enfors, S.O., Hecker, M. Biotechnol. Bioeng. (1999) [Pubmed]
  5. Expression of the pfl gene and resulting metabolite flux distribution in nuo and ackA-pta E. coli mutant strains. Singh, R., Yang, Y.T., Lu, B., Bennett, G.N., San, K.Y. Biotechnol. Prog. (2006) [Pubmed]
  6. Redistribution of metabolic fluxes in the central aerobic metabolic pathway of E. coli mutant strains with deletion of the ackA-pta and poxB pathways for the synthesis of isoamyl acetate. Dittrich, C.R., Vadali, R.V., Bennett, G.N., San, K.Y. Biotechnol. Prog. (2005) [Pubmed]
  7. Paired termini stabilize antisense RNAs and enhance conditional gene silencing in Escherichia coli. Nakashima, N., Tamura, T., Good, L. Nucleic Acids Res. (2006) [Pubmed]
  8. Ribose utilization in Lactobacillus sakei: analysis of the regulation of the rbs operon and putative involvement of a new transporter. Stentz, R., Zagorec, M. J. Mol. Microbiol. Biotechnol. (1999) [Pubmed]
 
WikiGenes - Universities