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Gene Review

chiA  -  periplasmic endochitinase

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK3325, JW3300, yheB
 
 
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Disease relevance of chiA

 

High impact information on chiA

 

Biological context of chiA

  • Deletion of the inverted-repeat sequences and curved DNA regions located in the upstream region of chiA essentially did not influence strain IC1270's chiA promoter activity in E. coli [5].
  • The gene coding for the major chitinase of Serratia marcescens, chiA, was cloned under the control of the tac promoter into the broad-host-range plasmid pKT240 and the integration vector pJFF350 [3].
  • The deduced amino acid sequence showed high similarity to those of bacterial chitinases classified into family 18 of glycosyl hydrolases. chiA was expressed in Escherichia coli and the recombinant chitinase (ChiA) was purified and examined [6].
 

Associations of chiA with chemical compounds

  • The gene-specific PCR primers were used to detect the presence of the chiA gene in strain C4 [7].

References

  1. The ChiA (YheB) protein of Escherichia coli K-12 is an endochitinase whose gene is negatively controlled by the nucleoid-structuring protein H-NS. Francetic, O., Badaut, C., Rimsky, S., Pugsley, A.P. Mol. Microbiol. (2000) [Pubmed]
  2. Molecular cloning, structural analysis, and expression in Escherichia coli of a chitinase gene from Enterobacter agglomerans. Chernin, L.S., De la Fuente, L., Sobolev, V., Haran, S., Vorgias, C.E., Oppenheim, A.B., Chet, I. Appl. Environ. Microbiol. (1997) [Pubmed]
  3. Introduction of the Serratia marcescens chiA gene into an endophytic Pseudomonas fluorescens for the biocontrol of phytopathogenic fungi. Downing, K.J., Thomson, J.A. Can. J. Microbiol. (2000) [Pubmed]
  4. Purification, characterization, and primary structure of a chitinase from Pseudomonas sp. YHS-A2. Lee, H.S., Han, D.S., Choi, S.J., Choi, S.W., Kim, D.S., Bai, D.H., Yu, J.H. Appl. Microbiol. Biotechnol. (2000) [Pubmed]
  5. Activity of Serratia plymuthica IC1270 gene chiA promoter region in Escherichia coli mutants deficient in global regulators of transcription. Khmel, I.A., Ovadis, M.I., Mayatskaya, A.V., Veselovskii, A.M., Bass, I.A., Lipasova, V.A., Bolshoy, A., Chet, I., Chernin, L.S. J. Basic Microbiol. (2005) [Pubmed]
  6. Cloning, Expression, and Characterization of a Chitinase from the Chitinolytic Bacterium Aeromonas hydrophila Strain SUWA-9. Lan, X., Zhang, X., Hu, J., Shimosaka, M. Biosci. Biotechnol. Biochem. (2006) [Pubmed]
  7. Cloning and Expression of a Chitinase Gene from Sanguibacter sp. C4. Tao, Y., Jin, H., Long, Z.F., Zhang, L., Ding, X.Q., Tao, K., Liu, S.G. Yi Chuan Xue Bao (2006) [Pubmed]
 
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