The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
Gene Review

crp  -  cAMP-activated global transcription factor...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK3345, JW5702, cap, csm
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of crp


High impact information on crp

  • Cyclic AMP exerted a strong negative control on the biosynthesis and of this enzyme for which the integrity of both the cya and the crp gene functions was necessary [3].
  • Using Western blotting and/or SDS-PAGE analysis, we demonstrate that glucose lowers the cellular concentration of CRP* through a reduction in crp* mRNA levels [4].
  • We have isolated several mutations in the crp gene that confer a CRP* phenotype [4].
  • These data strongly suggest that the lowered level of CRP* caused by glucose mediates catabolite repression in cya- crp* cells and that the autoregulatory circuit of the crp gene is involved in the down-regulation of CRP* expression by glucose [4].
  • CRP-cAMP-dependent operons of Escherichia coli can be expressed in cells lacking functional adenylate cyclase when they carry a second-site mutation in the crp gene (crp*) [4].

Chemical compound and disease context of crp


Biological context of crp


Associations of crp with chemical compounds

  • Growth of the csm strains on PTS (phosphoenolpyruvate phosphotransferase system) and non-PTS substrates was inhibited by 5 mM cAMP [2].
  • All csm strains were sensitive to catabolite repression mediated by alpha-methylglucoside [2].
  • The csm strains were found to accumulate toxic levels of methylglyoxal when grown on non-PTS substrates in the presence of exogenous cAMP [2].
  • Both cya and crp mutants are known to be resistant to mecillinam, an antibiotic which inhibits penicillin-binding protein 2 (involved in lateral wall elongation) and also affects septation [8].
  • Following i.t. inoculation, group scores based on pathologic and bacteriologic findings were 51%, 15%, and 9% for wild, delta cya, and delta crp O78 strains (inoculum approximately 2 x 10(7) organisms) and 98%, 31%, and 11%, respectively, for the corresponding O2 strains (inoculum approximately 4 x 10(6) organisms) [9].

Regulatory relationships of crp

  • In a strain carrying multiple copies of the crp gene and overproducing CAP the activity of adenylate cyclase is severely inhibited, although the in vivo rate of cAMP synthesis is similar to the parental strain [10].

Other interactions of crp

  • One class consisted of primary site revertants; a second class was pseudorevertants that had phenotypically reverted to wild type but retaining the original cya mutant and the third class of revertants, designated csm, were pseudorevertants hypersensitive to exogenous cAMP [2].
  • In addition, the expression of an aldB-lacZ fusion was decreased about 20-fold in the absence of crp [11].
  • The production of C51 microcin decreased or was absent in rpoS, crp and cya mutant cells [12].


  1. Cloning and sequence of the crp gene of Escherichia coli K 12. Cossart, P., Gicquel-Sanzey, B. Nucleic Acids Res. (1982) [Pubmed]
  2. Isolation and characterization of cAMP suppressor mutants of Escherichia coli K12. Melton, T., Snow, L.L., Freitag, C.S., Dobrogosz, W.J. Mol. Gen. Genet. (1981) [Pubmed]
  3. The acid phosphatase with optimum pH of 2.5 of Escherichia coli. Physiological and Biochemical study. Dassa, E., Cahu, M., Desjoyaux-Cherel, B., Boquet, P.L. J. Biol. Chem. (1982) [Pubmed]
  4. Glucose lowers CRP* levels resulting in repression of the lac operon in cells lacking cAMP. Tagami, H., Inada, T., Kunimura, T., Aiba, H. Mol. Microbiol. (1995) [Pubmed]
  5. Generation of deletions in the 3'-flanking sequences of the Escherichia coli crp gene that induce cyclic AMP suppressor functions. Barton, J.W., Melton, T. J. Bacteriol. (1987) [Pubmed]
  6. A new aspect of transcriptional control of the Escherichia coli crp gene: positive autoregulation. Hanamura, A., Aiba, H. Mol. Microbiol. (1992) [Pubmed]
  7. Cyclic AMP and its receptor protein are required for expression of transfer genes of conjugative plasmid F in Escherichia coli. Kumar, S., Srivastava, S. Mol. Gen. Genet. (1983) [Pubmed]
  8. Cyclic AMP and cell division in Escherichia coli. D'Ari, R., Jaffé, A., Bouloc, P., Robin, A. J. Bacteriol. (1988) [Pubmed]
  9. Construction and characterization of avian Escherichia coli cya crp mutants. Peighambari, S.M., Gyles, C.L. Avian Dis. (1998) [Pubmed]
  10. Multiple regulation of the activity of adenylate cyclase in Escherichia coli. Joseph, E., Bernsley, C., Guiso, N., Ullmann, A. Mol. Gen. Genet. (1982) [Pubmed]
  11. aldB, an RpoS-dependent gene in Escherichia coli encoding an aldehyde dehydrogenase that is repressed by Fis and activated by Crp. Xu, J., Johnson, R.C. J. Bacteriol. (1995) [Pubmed]
  12. Regulation of microcin C51 operon expression: the role of global regulators of transcription. Fomenko, D., Veselovskii, A., Khmel, I. Res. Microbiol. (2001) [Pubmed]
WikiGenes - Universities