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Gene Review

glmS  -  L-glutamine:D-fructose-6-phosphate...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK3722, JW3707
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Disease relevance of glmS

  • Insertion of transposon Tn7 into the Escherichia coli glmS transcriptional terminator [1].
  • Transducing phages carrying parts of the unc gene cluster were isolated: lambda uncA-9 and lambda glmS phages converted only some of the unc- mutants to the Unc+, as determined by complementation assays [2].
  • It was demonstrated that the class 2 integron was located in the Tn7 region inside the attTn7 locus downstream of glmS in Shigella [3].

High impact information on glmS

  • By deletion analysis of M13mp::attTn7, we show that attTn7 is contained within a 64 base-pair region; sequences adjacent to the actual insertion site and encoding the carboxy terminus of the glmS gene are required [4].
  • The gene, which we called glmR, is located between the glmS (PA5549) and glmU (PA5552) homologues of E. coli, responsible for the synthesis of UDP-N-acetylglucosamine-1-P, a precursor of both lipopolysaccharide (LPS) and peptidoglycan [5].
  • We showed that GlmR represses the transcription of the adjacent glmS homologue (PA5549) in P. aeruginosa, possibly affecting the pool of precursors for peptidoglycan and LPS synthesis [5].
  • This unique site is within a region of dyad symmetry that we have demonstrated to be the transcriptional terminator of the glmS gene which encodes the glutamine amidotransferase, glucosamine synthetase [1].
  • Hairpin loop structures resembling known rho-independent transcription termination signals are evident following the unc operon and glmS [6].

Chemical compound and disease context of glmS

  • NodM exhibits homology to amidotransferases, primarily to the D-glucosamine synthetase encoded by the glmS gene of Escherichia coli [7].
  • Cell-free extracts of E. coli carrying the glmS gene showed glutamate mutase activity which was strictly dependent on the addition of coenzyme B12 and component E purified from C. cochlearium [8].

Biological context of glmS

  • Transposition of Tn7 abolishes termination of glmS transcription at this site; the transcripts now extend into the left end of Tn7 and terminate at a new site, tm, 127 base pairs from the left end of Tn7 [1].
  • This plasmid complements the glmS mutation [9].
  • The gene encoding component S (glmS) was cloned in Escherichia coli and its nucleotide sequence was determined [8].
  • The complete gene cluster was sequenced in strain 789 (lpf789), where it is located between the genes glmS and pstS, and contains four ORFs, lpfA to lpfD [10].

Associations of glmS with chemical compounds


Other interactions of glmS


Analytical, diagnostic and therapeutic context of glmS


  1. Insertion of transposon Tn7 into the Escherichia coli glmS transcriptional terminator. Gay, N.J., Tybulewicz, V.L., Walker, J.E. Biochem. J. (1986) [Pubmed]
  2. Organization of unc gene cluster of Escherichia coli coding for proton-translocating ATPase of oxidative phosphorylation. Kanazawa, H., Tamura, F., Mabuchi, K., Miki, T., Futai, M. Proc. Natl. Acad. Sci. U.S.A. (1980) [Pubmed]
  3. Molecular characteristics of class 1 and class 2 integrons and their relationships to antibiotic resistance in clinical isolates of Shigella sonnei and Shigella flexneri. Pan, J.C., Ye, R., Meng, D.M., Zhang, W., Wang, H.Q., Liu, K.Z. J. Antimicrob. Chemother. (2006) [Pubmed]
  4. Genetic analysis of attTn7, the transposon Tn7 attachment site in Escherichia coli, using a novel M13-based transduction assay. Qadri, M.I., Flores, C.C., Davis, A.J., Lichtenstein, C.P. J. Mol. Biol. (1989) [Pubmed]
  5. Selection of an antibiotic-hypersusceptible mutant of Pseudomonas aeruginosa: identification of the GlmR transcriptional regulator. Ramos-Aires, J., Plésiat, P., Kocjancic-Curty, L., Köhler, T. Antimicrob. Agents Chemother. (2004) [Pubmed]
  6. DNA sequence around the Escherichia coli unc operon. Completion of the sequence of a 17 kilobase segment containing asnA, oriC, unc, glmS and phoS. Walker, J.E., Gay, N.J., Saraste, M., Eberle, A.N. Biochem. J. (1984) [Pubmed]
  7. Six nodulation genes of nod box locus 4 in Rhizobium meliloti are involved in nodulation signal production: nodM codes for D-glucosamine synthetase. Baev, N., Endre, G., Petrovics, G., Banfalvi, Z., Kondorosi, A. Mol. Gen. Genet. (1991) [Pubmed]
  8. Cloning, sequencing and expression in Escherichia coli of the gene encoding component S of the coenzyme B12-dependent glutamate mutase from Clostridium cochlearium. Zelder, O., Beatrix, B., Buckel, W. FEMS Microbiol. Lett. (1994) [Pubmed]
  9. Molecular cloning and overexpression of the glucosamine synthetase gene from Escherichia coli. Dutka-Malen, S., Mazodier, P., Badet, B. Biochimie (1988) [Pubmed]
  10. The lpf operon of invasive Escherichia coli. Ideses, D., Biran, D., Gophna, U., Levy-Nissenbaum, O., Ron, E.Z. Int. J. Med. Microbiol. (2005) [Pubmed]
  11. Characterization of Stg fimbriae from an avian pathogenic Escherichia coli O78:K80 strain and assessment of their contribution to colonization of the chicken respiratory tract. Lymberopoulos, M.H., Houle, S., Daigle, F., Léveillé, S., Brée, A., Moulin-Schouleur, M., Johnson, J.R., Dozois, C.M. J. Bacteriol. (2006) [Pubmed]
  12. Directed evolution and characterization of Escherichia coli glucosamine synthase. Deng, M.D., Grund, A.D., Wassink, S.L., Peng, S.S., Nielsen, K.L., Huckins, B.D., Burlingame, R.P. Biochimie (2006) [Pubmed]
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