Disease relevance of creC

• The phoM gene is one of the positive regulatory genes for the phosphate regulon of Escherichia coli [1].
• A phoM-lacZ protein fusion was constructed by using a plasmid carrying the phoM gene and a derivative of phage lambda, lambda plac Mu2 [2].

High impact information on creC

• No effect of phosphate concentration on cet or phoM gene expression could be found and thus their primary function may not be connected to the phosphate regulon [3].
• PhoM1206 could also transphosphorylate the product of phoM-orf2, which is structurally homologous to phoB and located immediately upstream of phoM [4].
• By molecular cloning of the wild-type phoM operon onto a multicopy plasmid and recombining onto the plasmid the pho-510 mutation that abolishes variation, the phoM operon, rather than some nearby gene, was shown to control variation [5].
• However, phoM operon expression was unaffected when tested with phoM operon lacZ transcriptional fusions [6].
• These data are consistent with the wild-type phoM operon having a negative, as well as a positive, regulatory role in gene expression [6].

Chemical compound and disease context of creC

• Plasmids containing the chromosome region of Escherichia coli encoding phoM, whose product is a positive regulator of alkaline phosphatase expression, were isolated from the Clarke and Carbon plasmid bank [2].

Biological context of creC

• We analyzed the nucleotide sequence of a 4.7-kilobase chromosomal DNA segment that encompasses the phoM gene and its flanking regions [1].
• Nucleotide sequence of the phoM region of Escherichia coli: four open reading frames may constitute an operon [1].
• Analysis of strains bearing the fusion on a multiple-copy plasmid or integrated at the lambda attachment site of the chromosome indicated that the synthesis of the phoM gene product was unaffected by phosphate limitation of growth [2].
• In addition, the switching Bap phenotype was not associated with an alternation in phoM operon expression [6].
• Restriction endonuclease analysis and in vitro mutagenesis of the hybird plasmids enabled the localization of the phoM gene locus to 3 kilobases of the cloned chromosomal fragment [2].

Associations of creC with chemical compounds

• The switching is regulated by the phoM operon and the presence of glucose; the pho-510 mutant form of the phoM operon abolishes both BAP clonal variation and the effect of glucose (B.L. Wanner, J. Bacteriol. 169:900-903, 1987) [6].

Analytical, diagnostic and therapeutic context of creC

• Molecular cloning of the wild-type phoM operon in Escherichia coli K-12 [5].
• Complementation tests indicated that the wild-type phoM allele is dominant to the pho-510 mutation when both are in single copy, but whichever allele is present in higher copy appears as dominant when multicopy plasmids are examined [5].

References