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Gene Review

phoB  -  response regulator in two-component...

Escherichia coli str. K-12 substr. MG1655

Synonyms: ECK0393, JW0389, phoRc
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Disease relevance of phoB


High impact information on phoB

  • Such induction was not significantly affected by mutations either in phoB (encoding the transcriptional regulator for the phosphate regulon) or rpoS (encoding a putative stationary phase-specific sigma factor) [3].
  • Comparison of the deduced amino acid sequence of narL to that of other bacterial genes revealed significant homologies to the phoB, ompR, and virG gene products based on the presence of similar protein domains [4].
  • Mutations causing three different phenotypes (previously reported as phoRa, phoRb, phoRc (Garen and Echols 1962a,b) are clustered in three closely linked genetic loci [5].
  • In the present report, the lacZ and phoB sites, which are 51 kb apart in the E. coli genome, were used for the introduction of the FRT/oriV sites [6].
  • The phoA gene (formerly called phoAIV) and the phoB gene (formerly called phoAIII) products have both been isolated from phosphate-starved cells, and a mutation in either gene decreased the total APase expressed under phosphate starvation conditions [7].

Biological context of phoB

  • We have determined the nucleotide sequence of the chromosomal segment containing the promoter and the coding region of the phoB gene [1].
  • We propose a model for PHO regulation in B. subtilis whereby the phoPR operon is transcribed in response to limiting phosphate concentration, resulting in activation of the PHO regulon transcription, including transcription of phoA and phoB [7].
  • Mutations in genes which are required for phosphorylation of Spo0A, spo0B and spo0F, also resulted in phoA and phoB hyperinduction, suggesting that phosphorylation of Spo0A is required for the repression of both APases in wild-type strains [7].

Associations of phoB with chemical compounds

  • sn-Glycerol-3-phosphate (G3P) or glyceryl phosphoryl phosphodiesters, the substrates of the phoB-dependent Ugp transport system, when transported exclusively through this system, can serve as a sole source of phosphate but not as a sole source of carbon (H. Schweizer, M. Argast, and W. Boos, J. Bacteriol. 150:1154-1163, 1982) [8].

Other interactions of phoB

  • A phoB deletion mutant was constructed, and we demonstrated that expression of pstS was strictly dependent on the function of the PhoB protein [9].
  • The transcriptome profiles of the wild-type and the phoB mutant strains were compared at the time point showing the highest expression levels of the phoB and phoR genes under a P-limiting condition [10].


  1. Nucleotide sequence of the phoB gene, the positive regulatory gene for the phosphate regulon of Escherichia coli K-12. Makino, K., Shinagawa, H., Amemura, M., Nakata, A. J. Mol. Biol. (1986) [Pubmed]
  2. Bacillus subtilis PhoP binds to the phoB tandem promoter exclusively within the phosphate starvation-inducible promoter. Liu, W., Hulett, F.M. J. Bacteriol. (1997) [Pubmed]
  3. Phosphate starvation and low temperature as well as ultraviolet irradiation transcriptionally induce the Escherichia coli LexA-controlled gene sfiA. Dri, A.M., Moreau, P.L. Mol. Microbiol. (1993) [Pubmed]
  4. Nucleotide sequence of the narL gene that is involved in global regulation of nitrate controlled respiratory genes of Escherichia coli. Gunsalus, R.P., Kalman, L.V., Stewart, R.R. Nucleic Acids Res. (1989) [Pubmed]
  5. Genetic analysis of regulatory mutants of alkaline phosphatase of E. coli. Kreuzer, K., Pratt, C., Torriani, A. Genetics (1975) [Pubmed]
  6. A broad-host-range in vivo pop-out and amplification system for generating large quantities of 50- to 100-kb genomic fragments for direct DNA sequencing. Wild, J., Hradecná, Z., Pósfai, G., Szybalski, W. Gene (1996) [Pubmed]
  7. Sequential action of two-component genetic switches regulates the PHO regulon in Bacillus subtilis. Hulett, F.M., Lee, J., Shi, L., Sun, G., Chesnut, R., Sharkova, E., Duggan, M.F., Kapp, N. J. Bacteriol. (1994) [Pubmed]
  8. The pho regulon-dependent Ugp uptake system for glycerol-3-phosphate in Escherichia coli is trans inhibited by Pi. Brzoska, P., Rimmele, M., Brzostek, K., Boos, W. J. Bacteriol. (1994) [Pubmed]
  9. Regulation of the phosphate regulon of Escherichia coli: characterization of the promoter of the pstS gene. Kimura, S., Makino, K., Shinagawa, H., Amemura, M., Nakata, A. Mol. Gen. Genet. (1989) [Pubmed]
  10. Novel gene members in the Pho regulon of Escherichia coli. Baek, J.H., Lee, S.Y. FEMS Microbiol. Lett. (2006) [Pubmed]
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