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pol  -  P2

Pseudomonas phage phi8

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Disease relevance of phi-8Lp3


High impact information on phi-8Lp3

  • Here we propose a new general method for directly determining RNA sequence based on the use of the RNA-dependent RNA polymerase from bacteriophage phi6 and the chain terminators (RdRP sequencing) [6].
  • The viral envelope encloses a nucleocapsid, consisting of an RNA-dependent RNA polymerase complex within an icosahedral shell assembled from approximately 800 copies of a 16 kDa subunit (protein P8, encoded by viral gene 8) [7].
  • In the amino acid sequence of the viral RNA-dependent RNA polymerase (P2), similarity was found to the comparable proteins of phi6, phi8, and phi13 [8].
  • This NTP-binding site is probably different from that of the unspecific viral NTPase found in protein P4 and also from that of the rNTP-specific RNA polymerase active site [9].
  • Procapsids can be restricted to minus-strand synthesis by high concentrations of CaCl2 or low concentrations of nucleotides, enabling us to separate the viral minus-strand (replication) and plus-strand (transcription) RNA-dependent RNA polymerase activities in vitro [10].

Analytical, diagnostic and therapeutic context of phi-8Lp3


  1. A mechanism for initiating RNA-dependent RNA polymerization. Butcher, S.J., Grimes, J.M., Makeyev, E.V., Bamford, D.H., Stuart, D.I. Nature (2001) [Pubmed]
  2. A novel virus-host cell membrane interaction. Membrane voltage-dependent endocytic-like entry of bacteriophage straight phi6 nucleocapsid. Poranen, M.M., Daugelavicius, R., Ojala, P.M., Hess, M.W., Bamford, D.H. J. Cell Biol. (1999) [Pubmed]
  3. Mutational analysis of the role of nucleoside triphosphatase P4 in the assembly of the RNA polymerase complex of bacteriophage phi6. Paatero, A.O., Mindich, L., Bamford, D.H. J. Virol. (1998) [Pubmed]
  4. Protein P7 of phage phi6 RNA polymerase complex, acquiring of RNA packaging activity by in vitro assembly of the purified protein onto deficient particles. Juuti, J.T., Bamford, D.H. J. Mol. Biol. (1997) [Pubmed]
  5. Packaging and replication regulation revealed by chimeric genome segments of double-stranded RNA bacteriophage phi6. Poranen, M.M., Bamford, D.H. RNA (1999) [Pubmed]
  6. Primer-independent RNA sequencing with bacteriophage phi6 RNA polymerase and chain terminators. Makeyev, E.V., Bamford, D.H. RNA (2001) [Pubmed]
  7. Assembly dynamics of the nucleocapsid shell subunit (P8) of bacteriophage phi6. Tuma, R., Bamford, J.K., Bamford, D.H., Thomas, G.J. Biochemistry (1999) [Pubmed]
  8. Characterization of phi12, a bacteriophage related to phi6: nucleotide sequence of the large double-stranded RNA. Gottlieb, P., Potgieter, C., Wei, H., Toporovsky, I. Virology (2002) [Pubmed]
  9. In vitro transcription of the double-stranded RNA bacteriophage phi 6 is influenced by purine NTPs and calcium. Ojala, P.M., Bamford, D.H. Virology (1995) [Pubmed]
  10. Differentiation between minus- and plus-strand synthesis: polymerase activity of dsRNA bacteriophage phi 6 in an in vitro packaging and replication system. van Dijk, A.A., Frilander, M., Bamford, D.H. Virology (1995) [Pubmed]
  11. Crystallization and preliminary X-ray crystallographic studies on the bacteriophage phi6 RNA-dependent RNA polymerase. Butcher, S.J., Makeyev, E.V., Grimes, J.M., Stuart, D.I., Bamford, D.H. Acta Crystallogr. D Biol. Crystallogr. (2000) [Pubmed]
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