Disease relevance of celA

• Transcriptional induction and expression of the endoglucanase celA gene from a ruminal Clostridium sp. ("C. longisporum") [1].
• Primer extension studies revealed that transcription of celA is initiated at different sites in Escherichia coli and Prevotella ruminicola [2].
• The nucleotide sequence of a 3.6 kb DNA fragment containing a cellodextrinase gene (celA) from Ruminococcus flavefaciens FD-1 was determined [3].
• Cloning and sequencing of beta-1,4-endoglucanase gene (celA) from Pseudomonas sp. YD-15 [4].
• Expression in Escherichia coli of the Myxococcus xanthus gene celA, which encodes an extracellular endoglucanase, resulted in CelA being distributed between cytoplasm, periplasm and membrane [5].

High impact information on celA

• The size of the celA mRNA transcript corresponded with the size of the open reading frame [1].
• Western blots (immunoblots), prepared by using a rabbit antiserum raised against CelA protein purified from Escherichia coli, revealed the extracellular location of CelA in Clostridium sp. Despite the absence of detectable celA mRNA, significant quantities of CelA were detected in the culture supernatant during growth on cellobiose [1].
• The size and location of the 5' end of celA mRNA encoding endoglucanase A of Clostridium thermocellum were investigated in C. thermocellum and in an Escherichia coli clone that carries and expresses the celA gene [6].
• An endoglucanase gene (celA) was isolated from a genomic library of the ruminal fungus Orpinomyces joyonii [7].
• One of the E. coli clones containing a Sau3AI fragment of Ecc genomic DNA hydrolyzed carboxymethyl cellulose and was shown to contain the 2.2 kb BamHI restriction fragment, which was subcloned to generate pLYCA100 named as celA [8].

Biological context of celA

• The deduced amino acid sequence of Endo-K had 30% homology with that of the celA enzyme from Clostridium thermocellum NCIB 10682 and 25% homology with that of the enzyme from Cellulomonas uda CB4 [9].
• Based on substrate specificity and catalytic mode, celA appears to encode a cellobiohydrolase, while celB and celC encode endoglucanases [10].

Associations of celA with chemical compounds

• Northern analysis of RNA extracted from R. albus 8 grown on cellulose indicated a celA transcript of approximately 2700 bases, whereas when R. albus 8 was grown on cellobiose, celA transcripts of approximately 3000 and 600 bases were detected [11].

Analytical, diagnostic and therapeutic context of celA

• T vectors with endoglucanase A (celA) gene for direct detection of PCR clones [12].

References