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Gene Review

BMVs1gp1  -  RNA1 translation product aa 1-961

Brome mosaic virus

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Disease relevance of BMVs1gp1

  • Using an RNA-dependent RNA polymerase (replicase) preparation from barley leaves infected with brome mosaic virus (BMV) to synthesize the viral subgenomic RNA in vitro, we now provide evidence that subgenomic RNA arises by internal initiation on the (-)-strand of genomic RNA [1].
  • We believe that this also represents the first in vitro demonstration of a replicase from a eukaryotic (+)-stranded RNA virus capable of initiating synthesis of (+)-sense RNA [1].
  • An RNA-dependent RNA polymerase (replicase) extract from brome mosaic virus-infected barley leaves has been shown to initiate synthesis of (-) sense RNA from (+) sense virion RNA [2].
  • Each deletion strain was transformed to express BMV replicase proteins and a BMV RNA replication template with the capsid gene replaced by a luciferase reporter [3].
  • Recent studies have shown that disrupted (open) rotavirus cores have an associated replicase activity which supports the synthesis of dsRNA from viral mRNA in a cell-free system (D. Chen, C. Q.-Y. Zeng, M. J. Wentz, M. Gorziglia, M. K. Estes, and R. F. Ramig, J. Virol. 68:7030-7039, 1994) [4].

High impact information on BMVs1gp1


Chemical compound and disease context of BMVs1gp1

  • Additional experiments involving coinoculation of either C3/DeltaCP-EGFP or C3/CP:EGFP with heterologous brome mosaic bromovirus (BMV) genomic RNAs 1 and 2 revealed that, in addition to movement protein and CP, viral replicase also influences cell-to-cell spread [9].

Biological context of BMVs1gp1

  • By native gel electrophoresis, enzymatic structure probing, and NMR spectroscopy it is shown here that in the absence of viral replicase the hexanucleotide loop 5'C1AUAG5A3' of this RNA structure can adopt a pseudo trinucleotide loop conformation by transloop base pairing between C1 and G5 [10].
  • Several sequences encoding functions, including replicase binding, initiation of (-) strand synthesis and tyrosine esterification are known to be nested within this region [11].

Anatomical context of BMVs1gp1


Associations of BMVs1gp1 with chemical compounds

  • Biochemical studies of the 5'AUG3' loop with various substitutions in the context of the whole SLC construct confirm that the clamped adenine motif exists in S-AUG remains a primary structural feature required for RNA synthesis by the BMV replicase [13].

Analytical, diagnostic and therapeutic context of BMVs1gp1


  1. Synthesis of brome mosaic virus subgenomic RNA in vitro by internal initiation on (-)-sense genomic RNA. Miller, W.A., Dreher, T.W., Hall, T.C. Nature (1985) [Pubmed]
  2. Minus-strand initiation by brome mosaic virus replicase within the 3' tRNA-like structure of native and modified RNA templates. Miller, W.A., Bujarski, J.J., Dreher, T.W., Hall, T.C. J. Mol. Biol. (1986) [Pubmed]
  3. Systematic, genome-wide identification of host genes affecting replication of a positive-strand RNA virus. Kushner, D.B., Lindenbach, B.D., Grdzelishvili, V.Z., Noueiry, A.O., Paul, S.M., Ahlquist, P. Proc. Natl. Acad. Sci. U.S.A. (2003) [Pubmed]
  4. Rotavirus VP1 alone specifically binds to the 3' end of viral mRNA, but the interaction is not sufficient to initiate minus-strand synthesis. Patton, J.T. J. Virol. (1996) [Pubmed]
  5. Mutant viral RNAs synthesized in vitro show altered aminoacylation and replicase template activities. Dreher, T.W., Bujarski, J.J., Hall, T.C. Nature (1984) [Pubmed]
  6. Modulation of replication, aminoacylation and adenylation in vitro and infectivity in vivo of BMV RNAs containing deletions within the multifunctional 3' end. Bujarski, J.J., Ahlquist, P., Hall, T.C., Dreher, T.W., Kaesberg, P. EMBO J. (1986) [Pubmed]
  7. Identification of sequences in Brome mosaic virus replicase protein 1a that mediate association with endoplasmic reticulum membranes. den Boon, J.A., Chen, J., Ahlquist, P. J. Virol. (2001) [Pubmed]
  8. RNA sequence and secondary structural determinants in a minimal viral promoter that directs replicase recognition and initiation of genomic plus-strand RNA synthesis. Sivakumaran, K., Kim, C.H., Tayon, R., Kao, C.C. J. Mol. Biol. (1999) [Pubmed]
  9. Capsid protein gene and the type of host plant differentially modulate cell-to-cell movement of cowpea chlorotic mottle virus. Rao, A.L., Cooper, B. Virus Genes (2006) [Pubmed]
  10. The Brome mosaic virus subgenomic promoter hairpin is structurally similar to the iron-responsive element and functionally equivalent to the minus-strand core promoter stem-loop C. Joost Haasnoot, P.C., Olsthoorn, R.C., Bol, J.F. RNA (2002) [Pubmed]
  11. Analysis of brome mosaic virus replication and aminoacylation functions by site-specific mutagenesis. Hall, T.C., Marsh, L.E., Dreher, T.W. J. Cell Sci. Suppl. (1987) [Pubmed]
  12. Brome mosaic virus replicase proteins localize with the movement protein at infection-specific cytoplasmic inclusions in infected barley leaf cells. Dohi, K., Mori, M., Furusawa, I., Mise, K., Okuno, T. Arch. Virol. (2001) [Pubmed]
  13. A mutant viral RNA promoter with an altered conformation retains efficient recognition by a viral RNA replicase through a solution-exposed adenine. Kim, C.H., Kao, C.C. RNA (2001) [Pubmed]
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