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Gene Review

BMVs2gp1  -  RNA-dependent RNA polymerase

Brome mosaic virus

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Disease relevance of BMVs2gp1

  • Using an RNA-dependent RNA polymerase (replicase) preparation from barley leaves infected with brome mosaic virus (BMV) to synthesize the viral subgenomic RNA in vitro, we now provide evidence that subgenomic RNA arises by internal initiation on the (-)-strand of genomic RNA [1].
  • This high resolution structure of an RNA promoter from a (+)-strand RNA virus provides new insights into how the RNA-dependent RNA polymerase binds to the RNA to initiate synthesis [2].
  • An RNA-dependent RNA polymerase (replicase) extract from brome mosaic virus-infected barley leaves has been shown to initiate synthesis of (-) sense RNA from (+) sense virion RNA [3].
  • We show that the RdRp of the plant-infecting BMV is capable of accurately, albeit inefficiently, initiating RNA synthesis from the subgenomic promoter of the animal-infecting Semliki Forest virus [4].
  • This observation and the established role of translation factors in bacteriophage Q beta RdRp suggest that association with translation factors may be a general feature of RNA replication by (+)-strand RNA viruses [5].

High impact information on BMVs2gp1


Chemical compound and disease context of BMVs2gp1


Biological context of BMVs2gp1

  • By means of in vitro replication assays using partially purified BMV RNA-dependent RNA polymerase (RdRp) it was found that other base pairs contribute to sg transcription, probably by stabilizing the formation of this pseudo triloop, which is proposed to be the primary element recognized by the viral replicase [11].
  • The nonstructural viral protein P1 was found to be a component of the RdRp complex (R. Quadt, H.J.M. Verbeek, and E.M.J. Jaspars, 1988, Virology 165, 256-261) [12].

Anatomical context of BMVs2gp1


Associations of BMVs2gp1 with chemical compounds

  • During the transition we were able to discern step-wise increases in the affinity of RdRp for RNA by measuring sensitivity to heparin and competition for RdRp by an alternative template [13].


  1. Synthesis of brome mosaic virus subgenomic RNA in vitro by internal initiation on (-)-sense genomic RNA. Miller, W.A., Dreher, T.W., Hall, T.C. Nature (1985) [Pubmed]
  2. RNA motifs that determine specificity between a viral replicase and its promoter. Kim, C.H., Kao, C.C., Tinoco, I. Nat. Struct. Biol. (2000) [Pubmed]
  3. Minus-strand initiation by brome mosaic virus replicase within the 3' tRNA-like structure of native and modified RNA templates. Miller, W.A., Bujarski, J.J., Dreher, T.W., Hall, T.C. J. Mol. Biol. (1986) [Pubmed]
  4. Sequence-specific recognition of a subgenomic RNA promoter by a viral RNA polymerase. Siegel, R.W., Adkins, S., Kao, C.C. Proc. Natl. Acad. Sci. U.S.A. (1997) [Pubmed]
  5. Characterization of a host protein associated with brome mosaic virus RNA-dependent RNA polymerase. Quadt, R., Kao, C.C., Browning, K.S., Hershberger, R.P., Ahlquist, P. Proc. Natl. Acad. Sci. U.S.A. (1993) [Pubmed]
  6. Initiation of genomic plus-strand RNA synthesis from DNA and RNA templates by a viral RNA-dependent RNA polymerase. Sivakumaran, K., Kao, C.C. J. Virol. (1999) [Pubmed]
  7. Spatial perturbations within an RNA promoter specifically recognized by a viral RNA-dependent RNA polymerase (RdRp) reveal that RdRp can adjust its promoter binding sites. Stawicki, S.S., Kao, C.C. J. Virol. (1999) [Pubmed]
  8. Initiation of minus-strand RNA synthesis by the brome mosaicvirus RNA-dependent RNA polymerase: use of oligoribonucleotide primers. Kao, C.C., Sun, J.H. J. Virol. (1996) [Pubmed]
  9. RNA-dependent RNA polymerase complex of Brome mosaic virus: analysis of the molecular structure with monoclonal antibodies. Dohi, K., Mise, K., Furusawa, I., Okuno, T. J. Gen. Virol. (2002) [Pubmed]
  10. Involvement of a nonstructural protein in the RNA synthesis of brome mosaic virus. Quadt, R., Verbeek, H.J., Jaspars, E.M. Virology (1988) [Pubmed]
  11. The Brome mosaic virus subgenomic promoter hairpin is structurally similar to the iron-responsive element and functionally equivalent to the minus-strand core promoter stem-loop C. Joost Haasnoot, P.C., Olsthoorn, R.C., Bol, J.F. RNA (2002) [Pubmed]
  12. Purification and characterization of brome mosaic virus RNA-dependent RNA polymerase. Quadt, R., Jaspars, E.M. Virology (1990) [Pubmed]
  13. RNA synthesis by the brome mosaic virus RNA-dependent RNA polymerase: transition from initiation to elongation. Sun, J.H., Kao, C.C. Virology (1997) [Pubmed]
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