Disease relevance of Bunyamwera virus

• No Bunyamwera virus RNA was detected in control transfections, but in cells which had previously been infected with recombinant vaccinia viruses expressing the L protein, both positive- and negative-sense Bunyamwera virus S segment RNA was detected [1].
• The expressed L protein was shown to be functional in synthesizing Bunyamwera virus RNA in a nucleocapsid transfection assay: recombinant vaccinia virus-infected cells were transfected with purified Bunyamwera virus nucleocapsids, and subsequently, total cellular RNA was analyzed by Northern (RNA) blotting [1].
• As in the virion, the small envelope protein (P28) of Belmont virus was not glycosylated, whereas the large envelope protein G107, and the corresponding G1 and G2 of Bunyamwera virus, were labelled intracellularly in mannose, galactose and glucosamine [2].

High impact information on Bunyamwera virus

• In addition, the abrogation of NSs expression changed Bunyamwera virus from a noninducer to an inducer of an interferon-beta promoter [3].
• Activation of PKR by Bunyamwera virus is independent of the viral interferon antagonist NSs [4].
• The recombinants had the G protein of the Maguari virus parent and the N protein of the Bunyamwera virus parent [5].
• Seventy-seven temperature-sensitive (ts) mutants belonging to three antigenically distinct and geographically isolated members of the Bunyamwera complex--Batai virus, Bunyamwera virus, and Maguari virus--have been isolated after 5-fluorouracil treatment [6].
• The Bunyamwera virus nonstructural protein NSs inhibits viral RNA synthesis in a minireplicon system [7].

Chemical compound and disease context of Bunyamwera virus

• The persistent virus interfered strongly with the replication of standard Bunyamwera virus in normal C6/36 cells and to a much lesser extent in BHK cells [8].
• Under conditions in which the envelope glycoproteins G1 and G2 of Bunyamwera virus were labelled in glucosamine, only G107 of Belmont virus was labelled [9].
• The nucleotide and amino acid sequences of the Bunyamwera virus M segment have been compared with those of the snowshoe hare virus M segment (Y. Eshita and D. H. L. Bishop, Virology 137, 227-240, 1984) [10].
• Cache Valley virus was isolated from the blood of a clinically normal horse in St Joseph County in September [11].
• The complete 4463 nucleotide sequence for the medium segment viral RNA of Cache Valley virus has been cloned and sequenced in four isolates; in addition, the G1 glycoprotein extracellular coding domains are completed for nine additional isolates, including two subtypes, Ft [12].

Biological context of Bunyamwera virus

• Mutagenesis of the L protein encoded by Bunyamwera virus and production of monospecific antibodies [13].
• Comparison of the nucleotide sequence to the prototype Bunyamwera virus sequence shows an identity of 63%, indicating several differences exist within the individual coding regions, most notably within the NSm and G1 coding regions [14].

Anatomical context of Bunyamwera virus

• The intracellular localisation of Bunyamwera virus NSs was investigated and found to be predominantly cytoplasmic, but intranuclear inclusion was also detected [7].

Gene context of Bunyamwera virus

• The suitability of this system to delineate functional domains within the Bunyamwera virus L protein is discussed [1].
• Cache Valley virus N expressed with a histidine tag, bovine serum albumin, and the basic lysozyme protein did not shift the IBV RNA [15].

References