The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Expression of cdc2 and cyclin B1 in Helicobacter pylori-associated gastric MALT and MALT lymphoma : relationship to cell death, proliferation, and transformation.

Mucosa-associated lymphoid tissue (MALT) may accumulate within gastric mucosa as a result of long standing Helicobacter pylori infection, and this acquired MALT may eventually develop into low-grade B-cell MALT lymphoma. To determine the possible association of cell cycle regulatory proteins and apoptotic cell death in the transformation of H. pylori gastritis to MALT lymphoma, the extent of cell proliferation, cell viability, expression of Cdc2/Cdk1 and cyclin B in gastric mucosal from patients with H. pylori-positive chronic gastritis (n = 7), MALT (n = 12), or MALT lymphoma (n = 12) were undertaken. Control tissue was obtained from H. pylori- negative patients (n = 5). Proliferating cell nuclear antigen (PCNA), Cdc2, and cyclin B1 were examined in paraffin embedded tissue by immunohistochemistry, while the apoptotic index (AI) was determined using the TUNEL assay. H&E staining for histology and modified Giemsa staining for the detection of H. pylori was conducted simultaneously. When compared to chronic gastritis tissue, those with MALT or MALT lymphoma had an increase in PCNA labeling index of 3.3- and 2.7-fold, while that for Cdc2/Cdk1 increased 2.3- and 3.1-fold, respectively. cyclin B1 labeling was 1.9 and 3.0 fold, while the AI was 3.4- and 1.4-fold higher in MALT and MALT lymphoma tissue, respectively, in the same comparison. On the other hand, the AI index of MALT lymphoma was 2. 5-fold lower than that for MALT tissues. The labeling scores for Cdc2/Cdk1 and cyclin B1 were significantly higher in the germinal center when compared to the mantle and marginal zones of MALT tissues. Using chi(2) and Pearson/Spearman's rho correlation coefficient with regression analyses, there was an inverse correlation between the AI and Cdc2/Cdk1 or cyclin B1 in MALT and MALT lymphoma tissues. There was no correlation between AI and PCNA labeling in any of the tissues. These results suggest that Cdc2/Cdk1 and cyclin B1 expression may be actively associated in the modulation of cellular death by apoptosis, as well as cellular proliferation and transformation during the evolution of H. pylori-associated gastritis to MALT lymphoma. Subclassification of high labeling score (>/=40) for Cdc2/Cdk1 and cyclin B1 and low labeling index (<0.6) for apoptotic cells in H. pylori-associated MALT may help in identifying a population of patients with an increased risk of developing MALT lymphoma.[1]


WikiGenes - Universities