The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Adenosine-induced apoptosis in glomerular mesangial cells.

BACKGROUND: Mesangial cell apoptosis is a mechanism of resolution of glomerular hypercellularity in inflammatory forms of glomerular injury in which adenosine (ADO) was shown to play an anti-inflammatory role. This, and the observation that mesangial cell have ADO receptors prompted us to determine whether ADO induces mesangial cell apoptosis and to explore underlying mechanisms. METHODS: Cultured mouse mesangial cell were incubated in the presence or absence of ADO or ADO receptor agonists (R-PIA, NECA, IB-MECA, CGS26180) or antagonists (DPCPX, DPSPX, MRS1191) for 48 hours. Cell death was assessed by trypan blue exclusion analysis. Apoptosis was assessed by DNA fragmentation, TUNEL staining and flow cytometry. RESULTS: ADO and the A3 ADO receptor agonist IB-MECA induced mesangial cell death, which was markedly attenuated by the A3 receptor antagonist MRS1191. The A1 receptor agonist R-PIA, A2 receptor agonist NECA or the A2a receptor agonist CGS-12680 had no effect. The IB-MECA-induced mesangial cell death was due to apoptosis. This occurred via a cAMP independent mechanism. RT-PCR analysis revealed presence of A3, A1 and A2b but lack of A2a receptor transcripts in MC total RNA. Western blot analysis of mesangial cell lysates revealed expression the A3 receptor protein only. CONCLUSION: The observations indicate that ADO induces mesangial cell apoptosis via stimulation of the A3 receptor.[1]


  1. Adenosine-induced apoptosis in glomerular mesangial cells. Zhao, Z., Kapoian, T., Shepard, M., Lianos, E.A. Kidney Int. (2002) [Pubmed]
WikiGenes - Universities