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Chemical Compound Review

LS-187087     (3Z)-5-amino-3-[[4-[4-[(2E)- 2-(8-amino-1...

Synonyms: AC1NV8R6
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Disease relevance of TRYPAN BLUE


Psychiatry related information on TRYPAN BLUE

  • Control experiments using psychological stress in adult rats as a means to transiently disrupt the BBB revealed that an increase in Trypan blue leakage correlated well with the disappearance of SMI71 immunoreactivity [6].
  • In the tail-flick assay, the P2 antagonists suramin (12-120 micrograms), Evans blue (0.1-10 micrograms), Trypan blue (1-30 micrograms) and Reactive blue 2 (1-30 micrograms) but not pyridoxalphosphate-6-azophenyl-2',4'-disulfonic acid (PPADS; 0.03-30 micrograms) caused moderate antinociception up to a doubling of the response latency [7].
  • Cell vitality of the untreated control groups and of the therapy group was determined 48 h after irradiation, using the trypan blue exclusion test [8].

High impact information on TRYPAN BLUE


Chemical compound and disease context of TRYPAN BLUE


Biological context of TRYPAN BLUE

  • Cell viability, as determined by trypan blue viability staining, was not influenced by the chemical treatment [19].
  • The isolated adipocytes thus obtained were suspended in Medium 199 and cultured at 37 degrees C. Cell viability was demonstrated in adipocytes cultured for up to 72 h by constancy of cell number, cell size, trypan-blue exclusion, and specific 125I-insulin binding [20].
  • Although defensin-treated K562 targets did not release chromium-labeled cytoplasmic components for 5-6 h, they experienced a rapid collapse (within minutes) of the membrane potential, efflux of rubidium, and influx of trypan blue [21].
  • These fragments were impermeable to trypan blue, still exhibited some metabolic activity such as the phosphorylation of AMP and TCN, but failed to replicate when the drug was removed [22].
  • At the same time, cell viability was determined by trypan blue exclusion and revealed median lethal doses (LD50) of 3.5 micrograms/ml (HL60), 15 micrograms/ml (Raji), 24 micrograms/ml (L1210), and 38 micrograms/ml (K562) [23].

Anatomical context of TRYPAN BLUE

  • Antibodydependent, complement-mediated cytotoxicity was demonstrated by the trypan blue test and Cr release assay for cultured ML cells, whereas no cytotoxicity was demonstrated for cells from B (SB) and T (MOLT 4) lymphoblastoid cell lines [24].
  • Only the uppermost epithelial cells in heavily labeled areas were devitalized as deduced by the morphologic appearance of the cells, the absence of labeling in the cells, the trypan blue exclusion test, and the trypsin digestion test [25].
  • MC-derived macrophages, activated with lymphokine elicited by the mitogens conconavalin A, phytohemagglutinin, or an amebic soluble protein preparation (strain HK9), killed 55% of amebae by 3 h in a trypan blue exclusion assay (P less than 0.001); during this time, 40% of the activated macrophages died [26].
  • Increased NO synthesis was associated with a parallel increase in myocyte death as measured by CPK release into the culture medium as well as by loss of membrane integrity, visualized by trypan blue staining [27].
  • This loss of adherence occurred while monocytes remained viable by criteria such as exclusion of trypan blue or release of lactate dehydrogenase [28].

Associations of TRYPAN BLUE with other chemical compounds


Gene context of TRYPAN BLUE


Analytical, diagnostic and therapeutic context of TRYPAN BLUE


  1. Exogenous immunoglobulin and the macrophage origin of Reed-Sternberg cells in Hodgkin's disease. Kadin, M.E., Stites, D.P., Levy, R., Warnke, R. N. Engl. J. Med. (1978) [Pubmed]
  2. Extracellular cytolysis by activated macrophages and granulocytes. I. Pharmacologic triggering of effector cells and the release of hydrogen peroxide. Nathan, C.F., Brukner, L.H., Silverstein, S.C., Cohn, Z.A. J. Exp. Med. (1979) [Pubmed]
  3. Antibody-mediated bacterial adhesion to cytomegalovirus-induced Fc receptors. Potential relationship to secondary infections complicating herpesvirus infections. Mackowiak, P.A., Marling-Cason, M., Smith, J.W., Luby, J.P. J. Clin. Invest. (1984) [Pubmed]
  4. Immunologic cytotoxicity against autologous human lymphocytes transformed or infected by Epstein-Bar virus: role of antibody-dependent cellular cytotoxicity in health individuals. Aya, T., Mizuno, F., Osato, T. J. Natl. Cancer Inst. (1980) [Pubmed]
  5. Claudin-4: a new target for pancreatic cancer treatment using Clostridium perfringens enterotoxin. Michl, P., Buchholz, M., Rolke, M., Kunsch, S., Löhr, M., McClane, B., Tsukita, S., Leder, G., Adler, G., Gress, T.M. Gastroenterology (2001) [Pubmed]
  6. Development of an intact blood-brain barrier in brain tissue transplants is dependent on the site of transplantation. Granholm, A.C., Curtis, M., Diamond, D.M., Branch, B.J., Heman, K.L., Rose, G.M. Cell transplantation. (1996) [Pubmed]
  7. Antinociceptive effect of intrathecally administered P2-purinoceptor antagonists in rats. Driessen, B., Reimann, W., Selve, N., Friderichs, E., Bültmann, R. Brain Res. (1994) [Pubmed]
  8. Response of human endometrium and ovarian carcinoma cell-lines to photodynamic therapy. Raab, G.H., Schneider, A.F., Eiermann, W., Gottschalk-Deponte, H., Baumgartner, R., Beyer, W. Arch. Gynecol. Obstet. (1990) [Pubmed]
  9. Halothane hepatitis. Detection of a constitutional susceptibility factor. Farrell, G., Prendergast, D., Murray, M. N. Engl. J. Med. (1985) [Pubmed]
  10. Calcium dependence of toxic cell death: a final common pathway. Schanne, F.A., Kane, A.B., Young, E.E., Farber, J.L. Science (1979) [Pubmed]
  11. Multiple sclerosis: Fas signaling in oligodendrocyte cell death. D'Souza, S.D., Bonetti, B., Balasingam, V., Cashman, N.R., Barker, P.A., Troutt, A.B., Raine, C.S., Antel, J.P. J. Exp. Med. (1996) [Pubmed]
  12. Differentiation in vitro of hybrid eosinophil/basophil granulocytes: autocrine function of an eosinophil developmental intermediate. Boyce, J.A., Friend, D., Matsumoto, R., Austen, K.F., Owen, W.F. J. Exp. Med. (1995) [Pubmed]
  13. Using cyclooxygenase-2 inhibitors as molecular platforms to develop a new class of apoptosis-inducing agents. Zhu, J., Song, X., Lin, H.P., Young, D.C., Yan, S., Marquez, V.E., Chen, C.S. J. Natl. Cancer Inst. (2002) [Pubmed]
  14. Cellular responses to Pyrularia thionin are mediated by Ca2+ influx and phospholipase A2 activation and are inhibited by thionin tyrosine iodination. Evans, J., Wang, Y.D., Shaw, K.P., Vernon, L.P. Proc. Natl. Acad. Sci. U.S.A. (1989) [Pubmed]
  15. Early midzonal cell death during low-flow hypoxia in the isolated, perfused rat liver: protection by allopurinol. Marotto, M.E., Thurman, R.G., Lemasters, J.J. Hepatology (1988) [Pubmed]
  16. Flavopiridol: a cytotoxic flavone that induces cell death in noncycling A549 human lung carcinoma cells. Bible, K.C., Kaufmann, S.H. Cancer Res. (1996) [Pubmed]
  17. The central role of sinusoidal endothelial cells in hepatic hypoxia-reoxygenation injury in the rat. Samarasinghe, D.A., Farrell, G.C. Hepatology (1996) [Pubmed]
  18. Prevention of cell death induced by tumor necrosis factor alpha in LNCaP cells by overexpression of sulfated glycoprotein-2 (clusterin). Sensibar, J.A., Sutkowski, D.M., Raffo, A., Buttyan, R., Griswold, M.D., Sylvester, S.R., Kozlowski, J.M., Lee, C. Cancer Res. (1995) [Pubmed]
  19. Inhibition of interferon gamma production by benzene and benzene metabolites. Cheung, S.C., Nerland, D.E., Sonnenfeld, G. J. Natl. Cancer Inst. (1988) [Pubmed]
  20. Regulation of lipoprotein lipase in primary cultures of isolated human adipocytes. Kern, P.A., Marshall, S., Eckel, R.H. J. Clin. Invest. (1985) [Pubmed]
  21. Mechanism of mammalian cell lysis mediated by peptide defensins. Evidence for an initial alteration of the plasma membrane. Lichtenstein, A. J. Clin. Invest. (1991) [Pubmed]
  22. Transport, phosphorylation, and toxicity of a tricyclic nucleoside in cultured Novikoff rat hepatoma cells and other cell lines and relase of its monophosphate by the cells. Plagemann, P.G. J. Natl. Cancer Inst. (1976) [Pubmed]
  23. Sensitivity of leukemia cell lines to cytotoxic alkyl-lysophospholipids in relation to O-alkyl cleavage enzyme activities. Unger, C., Eibl, H., Kim, D.J., Fleer, E.A., Kötting, J., Bartsch, H.H., Nagel, G.A., Pfizenmaier, K. J. Natl. Cancer Inst. (1987) [Pubmed]
  24. Cytotoxicity of antisera to a myelogenous leukemia cell line with the Philadelphia chromosome. Whitson, M.E., Lozzio, C.B., Lozzio, B.B., Wust, C.J., Sonoda, T., Avery, B. J. Natl. Cancer Inst. (1976) [Pubmed]
  25. Viability of the human cervical epithelium with dysplasia and carcinoma in situ. Rubio, C.A., Lagerlöf, B., Kock, Y., Söderberg, G., Thomassen, P. J. Natl. Cancer Inst. (1978) [Pubmed]
  26. Interaction of human leukocytes and Entamoeba histolytica. Killing of virulent amebae by the activated macrophage. Salata, R.A., Pearson, R.D., Ravdin, J.I. J. Clin. Invest. (1985) [Pubmed]
  27. The lethal effects of cytokine-induced nitric oxide on cardiac myocytes are blocked by nitric oxide synthase antagonism or transforming growth factor beta. Pinsky, D.J., Cai, B., Yang, X., Rodriguez, C., Sciacca, R.R., Cannon, P.J. J. Clin. Invest. (1995) [Pubmed]
  28. 1,25-Dihydroxyvitamin D3 maintains adherence of human monocytes and protects them from thermal injury. Polla, B.S., Healy, A.M., Amento, E.P., Krane, S.M. J. Clin. Invest. (1986) [Pubmed]
  29. Complement membrane attack complex stimulates production of reactive oxygen metabolites by cultured rat mesangial cells. Adler, S., Baker, P.J., Johnson, R.J., Ochi, R.F., Pritzl, P., Couser, W.G. J. Clin. Invest. (1986) [Pubmed]
  30. Association of tissue factor activity with the surface of cultured cells. Maynard, J.R., Heckman, C.A., Pitlick, F.A., Nemerson, Y. J. Clin. Invest. (1975) [Pubmed]
  31. Growth inhibition and induction of apoptosis by fenretinide in small-cell lung cancer cell lines. Kalemkerian, G.P., Slusher, R., Ramalingam, S., Gadgeel, S., Mabry, M. J. Natl. Cancer Inst. (1995) [Pubmed]
  32. Evaluation of University of Wisconsin cold-storage solution in warm hypoxic perfusion of rat liver: the addition of fructose reduces injury. Brass, C.A., Crawford, J.M., Narciso, J.P., Gollan, J.L. Gastroenterology (1993) [Pubmed]
  33. Protein secretion in suspensions of isolated rat hepatocytes: no influence of acute ethanol administration. Mørland, J., Rothschild, M.A., Oratz, M., Mongelli, J., Donor, D., Schreiber, S.S. Gastroenterology (1981) [Pubmed]
  34. Parathyroid hormone-Smad3 axis exerts anti-apoptotic action and augments anabolic action of transforming growth factor beta in osteoblasts. Sowa, H., Kaji, H., Iu, M.F., Tsukamoto, T., Sugimoto, T., Chihara, K. J. Biol. Chem. (2003) [Pubmed]
  35. Transcriptional repressor activating transcription factor 3 protects human umbilical vein endothelial cells from tumor necrosis factor-alpha-induced apoptosis through down-regulation of p53 transcription. Kawauchi, J., Zhang, C., Nobori, K., Hashimoto, Y., Adachi, M.T., Noda, A., Sunamori, M., Kitajima, S. J. Biol. Chem. (2002) [Pubmed]
  36. Apaf-1, Bcl-xL, cytochrome c, and caspase-9 form the critical elements for cerebral vascular protection by erythropoietin. Chong, Z.Z., Kang, J.Q., Maiese, K. J. Cereb. Blood Flow Metab. (2003) [Pubmed]
  37. A neutrophil-derived proteolytic inactive elastase homologue (hHBP) mediates reversible contraction of fibroblasts and endothelial cell monolayers and stimulates monocyte survival and thrombospondin secretion. Ostergaard, E., Flodgaard, H. J. Leukoc. Biol. (1992) [Pubmed]
  38. Down-regulation of BRCA1 and BRCA2 in human ovarian cancer cells exposed to adriamycin and ultraviolet radiation. Fan, S., Twu, N.F., Wang, J.A., Yuan, R.Q., Andres, J., Goldberg, I.D., Rosen, E.M. Int. J. Cancer (1998) [Pubmed]
  39. Isolation of colonic crypts that maintain structural and metabolic viability in vitro. Gibson, P.R., van de Pol, E., Maxwell, L.E., Gabriel, A., Doe, W.F. Gastroenterology (1989) [Pubmed]
  40. Cytosolic Ca2+ deregulation and blebbing after HgCl2 injury to cultured rabbit proximal tubule cells as determined by digital imaging microscopy. Smith, M.W., Phelps, P.C., Trump, B.F. Proc. Natl. Acad. Sci. U.S.A. (1991) [Pubmed]
  41. Load regulation of the properties of adult feline cardiocytes. The role of substrate adhesion. Cooper, G., Mercer, W.E., Hoober, J.K., Gordon, P.R., Kent, R.L., Lauva, I.K., Marino, T.A. Circ. Res. (1986) [Pubmed]
  42. p53 gene mutations are associated with decreased sensitivity of human lymphoma cells to DNA damaging agents. Fan, S., el-Deiry, W.S., Bae, I., Freeman, J., Jondle, D., Bhatia, K., Fornace, A.J., Magrath, I., Kohn, K.W., O'Connor, P.M. Cancer Res. (1994) [Pubmed]
  43. A timetable of events during programmed cell death induced by trophic factor withdrawal from neuronal PC12 cells. Mesner, P.W., Epting, C.L., Hegarty, J.L., Green, S.H. J. Neurosci. (1995) [Pubmed]
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