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Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)

Staphylococcal enterotoxin B inhibits the production of interleukin-4 in a human mast-cell line HMC-1.

Staphylococcal enterotoxins belong to the recently characterized group of immunocytotropic bacterial superantigens that are potent mitogens for human T cells. Superantigens are presented, but without intracellular processing, to T cells by monocyte/macrophages, Langerhans' cells and keratinocytes via the class II major histocompatibility complex (MHC) molecules. Superantigens have been demonstrated to act as potent inducers of several proinflammatory cytokines in the antigen-presenting cells such as interleukin-1 ( IL-1) and tumour necrosis factor-alpha (TNF-alpha). As mast cells participate in the pathogenesis of several inflammatory skin disorders such as atopic dermatitis (AD), which is often aggravated by staphylococcal infections, we studied the effect of staphylococcal enterotoxin B (SEB) superantigen on the histamine release and IL-4 expression in a human mast-cell line (HMC-1). Incubation of SEB (50 micrograms/ml) with HMC-1 cells for 45 min, could not induce any histamine release. The HMC-1 cells were also stimulated with various concentrations of SEB (0, 1, 10, 20, 50 micrograms/ml) for 1, 2, 3 and 4 days. Clear dose-dependent inhibition of IL-4 protein production and release was observed on day 4 without any observed effect on cell viability. Compared with unstimulated HMC-1 cells, after 50 micrograms/ml SEB stimulation, the IL-4 mRNA levels decreased steadily in the 2, 6, 18 and 24 hr samples in repeated experiments as measured with the reverse transcription polymerase chain reaction (RT-PCR) method. In comparison, a biphasic decrease in TNF-alpha expression was found. Our results show that an human leukaemic mast cells, superantigen stimulation downregulates the production of IL-4.[1]


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