Disease relevance of EINECS 207-429-4

• Models of the trisaccharide, 1-kestose [beta-D-fructofuranosyl-(2----1)-beta-D-fructofuranosyl-(2 in equilibrium with 1)-alpha-D -glucopyranoside], were analyzed with the molecular mechanics computer program MM2(87) to ascertain their inter-ring torsion angles, primary alcohol side-group orientations, and ring puckering [1].

High impact information on EINECS 207-429-4

• A comparison revealed that chicory 1-FFT has a high affinity for sucrose (Suc), fructose (Fru), and 1-kestose as acceptor substrate [2].
• When the cDNA was transiently expressed in tobacco protoplasts, the corresponding enzyme preparations produced 1-kestose from Suc, showing that the cDNA encodes a 1-SST [3].
• Interestingly, point mutations of the EC-motif resulted in an enzyme which had lost the capability to form 1-kestose and glucose from sucrose but still accepted 1-kestose, producing fructose and sucrose as well as nystose [4].
• It was also found that this enzyme catalyzed hydrolysis of such fructooligosaccharides as 1-kestose, nystose, and 1-fructosylnystose by liberating fructose [5].
• The hydrolysis kinetics of trisaccharides consisting of glucose, galactose, and fructose residues with different glycosidic bonds, 1-kestose, d-melezitose, d-raffinose, and lactosucrose, in subcritical water were conducted over the temperature range of 150-230 degrees C and at a constant pressure of 10 MPa [6].

Anatomical context of EINECS 207-429-4

• In the protoplast system, 1-kestose seemed to be the only fructan product of the 1-SST [7].
• The capacity for 1-kestose uptake into the vacuole of fructan storing Jerusalem artichoke tubers was investigated [8].

Associations of EINECS 207-429-4 with other chemical compounds

• They were characterized by acid hydrolysis and HPLC as 1-kestose, 6-kestose and neokestose [9].
• SST activity was characterized by the synthesis of 1-kestose from sucrose and FFT activity by the production of nystose from 1-kestose [10].
• 2. The enzyme activity was stable under 40 degrees C and the optimal temperature was 55 degrees C. This enzyme catalyzed the hydrolysis of sucrose, 1-kestose, nystose, inulin and raffinose at the relative velocities of 100, 297, 365, 140 and 3.8, respectively, but did not catalyze the hydrolysis of maltose or cellobiose [11].

Analytical, diagnostic and therapeutic context of EINECS 207-429-4

• No other studies of fructofuranoses have observed that crystallographic form, thus suggesting that the 18 hydrogen bonds created upon crystallization of 1-kestose support the ring deformation [1].

References