Disease relevance of 3-Dehydroecdysone

• A protein from the cabbage looper, Trichoplusia ni, regulated by a bacterial infection is homologous to 3-dehydroecdysone 3beta-reductase [1].

High impact information on 3-Dehydroecdysone

• Molecular cloning and characterization of hemolymph 3-dehydroecdysone 3beta-reductase from the cotton leafworm, Spodoptera littoralis. A new member of the third superfamily of oxidoreductases [2].
• The foregoing results, together with comparison of the metabolism of 3-dehydro[3H]ecdysone and [3H]ecdysone by the enzyme preparation in the presence of unlabelled ecdysone and NADPH, support the intermediacy of 3-dehydroecdysone in the 3-epimerization of ecdysone [3].
• The results suggest that the 3-dehydroecdysone 3 beta-reductase has a high-affinity (low Km) binding site for 3-dehydroecdysone substrate, together with a lower-affinity inhibition site [4].
• In the course of development, 3-dehydroecdysone 3beta-reductase mediates the conversion of 3-dehydroecdysone (3dE) secreted from the prothoracic glands to ecdysone (E), which is subsequently converted to 20-hydroxyecdysone (20E), the major insect molting hormone [1].
• When the expression of the various genes was studied in the epidermis in vitro, 20E and 3D20E had similar effects, whereas E, 3DE, 26-hydroxyecdysone and 20,26-dihydroxyecdysone were ineffective [5].

Biological context of 3-Dehydroecdysone

• The enzyme responsible for hydroxylation at C-25 of the putative 3-dehydroecdysone precursor, 14-hydroxy-5 beta-cholest-7-en-3,6-dione, is also microsomal, while those mediating hydroxylations at C-22 and C-2 of 3,14,25-trihydroxy-5 beta-cholest-7-en-6-one are mitochondrial [6].
• The prothoracic glands of the tobacco hornworm, Manduca sexta, respond to prothoracicotropic hormone (PTTH) by a regulatory pathway involving cAMP, protein phosphorylation, protein synthesis, and enhanced secretion of ecdysteroids including ecdysone and 3-dehydroecdysone [7].

Associations of 3-Dehydroecdysone with other chemical compounds

• Y-organs of the xanthid crab, Menippe mercenaria, secrete ecdysteroid hormones in vitro, apparently both 3-dehydroecdysone and 25-deoxyecdysone [8].

Gene context of 3-Dehydroecdysone

• When CG9504 was expressed in COS7 cells, significant conversion of ecdysone into 3-dehydroecdysone was observed [9].

Analytical, diagnostic and therapeutic context of 3-Dehydroecdysone

• The prothoracic glands (PGs) of Lymantria dispar (day-5 female, last-stage larvae) produce both ecdysone and an ecdysteroid which has the same retention time on reverse-phase liquid chromatography (RPLC) as a known standard of 3-dehydroecdysone [10].

References