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Chemical Compound Review

Isorubine     [4-[bis(4-amino-3-methyl...

Synonyms: Neofuchsine, Fuchsin- N, Fuchsin-?N, Fuchsin N, Fuchsin NB, ...
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Disease relevance of New fuchsine


High impact information on New fuchsine

  • Using either hexazotized pararosaniline or new fuchsin as coupling agents, we investigated the ultrastructural localization of alpha-naphthyl acetate esterase (ANAE) activity in guinea pig bone marrow and peritoneal exudates, and in human peripheral blood cells [2].
  • In the second step, the cells were incubated with alkaline phosphatase-conjugated murine monoclonal antibodies against a common cytokeratin epitope and a red color reaction was developed with new fuchsin as substrate [3].
  • For this reason, the APase/FR reaction was replaced by the APase/new fuchsin (NF, red colour) reaction and the washing steps after the PO/TMB reaction were restricted to the use of phosphate buffer pH 6 [4].
  • 0. Furthermore, to improve the efficiency of the ISH reaction, APase/NF was applied in an avidin-biotin complex detection system and, to avoid target shielding in the triple-target ISH, the third primary antibody was applied prior to the second enzyme cytochemical reaction [4].
  • The reaction with the new-fuchsin substrate gave a red precipitate, showing a specific and intense labelling in erythroblasts, polychromatophilic erythroblasts and myeloblasts [5].

Associations of New fuchsine with other chemical compounds

  • Similarly localized E600-resistant reaction product occurred with the acetate substrate and hexazotized New Fuchsin at pH 7.4, in the absence of taurocholate [6].

Gene context of New fuchsine

  • CEA and EMA were detected by the indirect method, and were colored by DAB, AEC, and new fuchsin [7].
  • In addition to immunoperoxidase, alkaline phosphatase-antialkaline phosphatase (APAAP) staining with naphthol ASBI phosphate and New Fuchsin as a substrate could be used [8].
  • METHODS--This double immunohistochemical staining method combines alkaline phosphatase-anti-alkaline phosphatase (APAAP) using New Fuchsin as a chromogen and indirect immunofluorescence [9].


  1. DNA cytometry in pleomorphic adenomas with cytologic atypia. Safali, M., Celasun, B., Günhan, O. Anal. Quant. Cytol. Histol. (2002) [Pubmed]
  2. Ultrastructural localization of nonspecific esterase activity in guinea pig and human monocytes, macrophages, and lymphocytes. Monahan, R.A., Dvorak, H.F., Dvorak, A.M. Blood (1981) [Pubmed]
  3. Immunoglobulin and enzyme-conjugated dextran polymers enhance u-PAR staining intensity of carcinoma cells in peripheral blood smears. Werther, K., Normark, M., Hansen, B.F., Nielsen, H.J. J. Histochem. Cytochem. (1999) [Pubmed]
  4. Multi-colour brightfield in situ hybridisation on tissue sections. Hopman, A.H., Claessen, S., Speel, E.J. Histochem. Cell Biol. (1997) [Pubmed]
  5. Evidence for a direct action of GH on haemopoietic cells of a marine fish, the gilthead sea bream (Sparus aurata). Calduch-Giner, J.A., Sitjà-Bobadilla, A., Alvarez-Pellitero, P., Pérez-Sánchez, J. J. Endocrinol. (1995) [Pubmed]
  6. Lipolytic and esterolytic activities in posterior lingual glands of rat: a histochemical study. Triantafyllou, A., Fletcher, D., Scott, J. Histochem. J. (2002) [Pubmed]
  7. Usefulness of Auto Cyto Fix (membrane filter method) for the application of immunohistochemistry. Iijima, J., Shiina, Y., Ohkoudo, M., Sakuma, K. Diagn. Cytopathol. (2002) [Pubmed]
  8. Combined immuno- and non-radioactive hybridocytochemistry on cells and tissue sections: influence of fixation, enzyme pre-treatment, and choice of chromogen on detection of antigen and DNA sequences. Mullink, H., Walboomers, J.M., Tadema, T.M., Jansen, D.J., Meijer, C.J. J. Histochem. Cytochem. (1989) [Pubmed]
  9. Improved double immunohistochemical staining method for cryostat and paraffin wax sections, combining alkaline phosphatase anti-alkaline phosphatase and indirect immunofluorescence. Tao, Q., Srivastava, G., Loke, S.L., Chan, E.Y., Ho, F.C. J. Clin. Pathol. (1994) [Pubmed]
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