Disease relevance of Nilverm

• Levamisole (Ketrax), and anthelmintic drug commonly used in the tropics, has been found to restore the impaired spontaneous rosette-forming lymphocytes and delayed cutaneous hypersensitivity reactions in experimentally malnourished rats [1].
• L-tetramisole affected the production of the physiological IFN, but not the production of IFN induced by Newcastle disease virus (NDV) in the peritoneal cells of mice or in L929 cells [2].

High impact information on Nilverm

• On the contrary, ATP concentrations did not affect vanadate inhibition of ATP hydrolysis by intact MVs if ALP activity was blocked by l-tetramisole [3].
• We sought to clarify its role in this process by studying the effect of L-tetramisole, a strong uncompetitive inhibitor of this enzyme, and D-tetramisole, its inactive isomer, on the uptake of 32Pi and 45Ca by matrix vesicle-enriched microsomes obtained from chicken epiphyseal cartilage [4].
• To clarify the role of alkaline phosphatase (ALP) and ATPase in skeletal mineralization, we studied the effect of the anthelmintic drug, l-tetramisole (levamisole), a stereospecific inhibitor of ALP activity, and its inactive isomer, d-tetramisole (dexamisole), on in vitro calcification of rachitic rat cartilage [5].
• L-tetramisole. Enhancement of human lymphocyte response to antigen [6].
• The anthelminthic drug L-Tetramisole enhanced in vitro lymphocyte transformation of normal young adults in the presence of PPD, Candida or measles antigen [6].

Biological context of Nilverm

• Additionally, [gamma-32P]ATP hydrolysis by purified ALP in contrast to that by intact vesicles was completely inhibited by l-tetramisole, a specific inhibitor of ALP, suggesting a loss of specific ATPase during purification [3].

Anatomical context of Nilverm

• Transmission electron microscopy showed that specific reaction product of the 5'-Nase after incubation in a medium containing L-tetramisole was predominantly localized on the outer surface of the lymphatic endothelial cell membranes [7].
• The isolated visualization of 5'-nucleotidase activity with a simultaneous inhibition of alkaline phosphatase with L-tetramisole is considered to be the best way to histochemically demonstrate lymphatic capillaries [8].

Associations of Nilverm with other chemical compounds

• ATPase, ADPase, and AMPase (5'-nucleotidase) were demonstrated with lead precipitation methods, L-tetramisole was used to inhibit unspecific alkaline phosphatase (alPase); alPase activities were shown with tetrazolium methods, using 5-bromo-4-chloro-3-indoxyl phosphate as substrate [9].

References