Disease relevance of mannosylretinylphosphate

• The formation of mannosyl retinyl phosphate appears a constitutive property and can be a mechanism by which retinoids induce early cell surface changes during differentiation of embryonal carcinoma cells [1].

High impact information on mannosylretinylphosphate

• Retinyl phosphate mannose synthesis in rat liver membranes. Phospholipase sensitivity and phospholipid requirement [2].
• The amount of mannosyl retinyl phosphate formed (pmol/min per mg of protein) for each fraction was: rough endoplasmic reticulum 0.48 +/- 0.09 (mean +/- S.D.); smooth membranes (consisting of 60% smooth endoplasmic reticulum and 40% Golgi apparatus), 0.18 +/- 0.03; Golgi apparatus, 0.13 +/- 0.03; and plasma membrane 0.02 [3].
• Mannosyl retinyl phosphate occurs, therefore, in vivo in liver and intestinal mucosa, and it is accompanied by a closely similar, though slightly less polar, compound that remains unidentified [4].
• A pH of 6.0-6.2 favored the formation of dolichyl mannosyl phosphate; a higher pH (6.7-7.0) that of mannosyl retinyl phosphate [5].
• The mannosyl derivative of phosphorylated vitamin A, mannosylretinylphosphate, is synthesized in vivo and in vitro by most epithelial tissues, including mouse epidermal cells [6].

Anatomical context of mannosylretinylphosphate

• Triple-labeled mannosylretinylphosphate was prepared from [3H]retinylphosphate, retinyl[32P]phosphate and GDP-[14C]mannose from incubations containing rat liver microsomes [7].
• In both A- and A-I- groups, thyroid cytosol 3H-RPM (retinyl-phosphate-mannose) and 3H-mannose incorporation into the core of the 12S-Tg and 19S-Tg species were reduced by 40-50% [8].

Associations of mannosylretinylphosphate with other chemical compounds

• These data suggest that retinol, as mannosylretinylphosphate, and retinoic acid, as mannosylretinoidphosphate, function as mannosyl carriers in biologic membranes [6].
• By means of DEAE-cellulose, silicic acid and thin-layer chromatography, the mannose-containing lipids were separated into mannosyl retinyl phosphate (MRP, Rf 0.2) and dolichyl mannosyl phosphate (DMP, Rf 0.4), respectively [9].

References