High impact information on Diphenylcarbazide

• However, B thylakoids possess a partially active PSII reaction center, as demonstrated by light-dependent reduction of silicomolybdate with 1,5-diphenylcarbazide (DPC) as an electron donor, and the presence of the PSII reaction center polypeptides of 44-47 kDa [1].
• Diphenylcarbazide, an efficient electron donor to Tyr-Z+ only in PSII membranes in which Mn is removed or dislocated, was found to donate electrons in Ca(2+)-depleted PSII, indicating altered accessibility or reactivity [2].
• Kinetic analyses were made of the effects of weak-light photoinhibition on the capacity of NH2OH-extracted photosystem II membranes to photooxidize the exogenous electron donors Mn2+, diphenylcarbazide, and I- or to assemble functional water-oxidizing complexes during photoactivation [3].
• Ca2+ stimulates electron transport from diphenylcarbazide to 2,6-dichloroindophenol catalysed by lead-inhibited cation-free preparations, showing the presence of two cation-binding sites in these particles [4].
• On-line preconcentration and determination of chromium(VI) in waters by high-performance liquid chromatography using precolumn complexation with 1,5-diphenylcarbazide [5].

Biological context of Diphenylcarbazide

• 0. The addition of either artificial electron donors for PSII such as MnCl2 or diphenylcarbazide, or diuron as an inhibitor of electron transfer from QA, the primary bound quinone acceptor, to QB, the secondary bound quinone acceptor of PSII, resulted in a decrease in oxygen consumption rate at basic pH to value close to ones measured at pH 6 [6].

Associations of Diphenylcarbazide with other chemical compounds

• NBD treatment also abolishes the EPR signal IIfast of oxidized tyrosine donor, Yz, and inhibits the electron transport from diphenylcarbazide to 2,6-dichlorophenol-indophenol [7].
• Photooxidation reactions of diphenylcarbazide and their DCMU-sensitivity in thylakoids of the blue-green alga Oscillatoria chalybea [8].
• The eluate was then acidified with hydrochloric acid and complexed with 1,5-diphenylcarbazide reagent prior to flow injection analysis [9].
• In thylakoids isolated from cadmium-treated plants a loss in the capability to reduce 2,6-dichlorophenolindophenol is observed, which is partially restored if diphenylcarbazide is used as an electron donor, indicating that cadmium affects water splitting activity [10].
• Chromic oxide content of the oxidized solution is then measured against known standards either directly by spectrophotometry in the visible range at 350, 370, or 440 nm or after forming a colored complex with diphenylcarbazide (DPC) by colorimetry at 540 nm [11].

Gene context of Diphenylcarbazide

• Chromate was preconcentrated on a C18 column (50 x 6 mm I.D.) after complexation with diphenylcarbazide (DPC) [5].
• This preparation retained photosystem II activity as evidenced by the photoreduction of DPIP in the presence of diphenylcarbazide (222 mumol DPIP/mg chlorophyll/h) [12].
• EDTA-washed particles retain the L-amino acid oxidase with high specificity for the basic L-amino acids [Pistorius, E. K. and Voss, H. (1980) Biochem. Biophys. Acta, 611, 227-240] as well as the ability to reduce 2,6-dichloroindophenol with diphenylcarbazide as a donor in the light [13].
• The reliability of the semi-automatic devices was verified by comparing the results obtained with those found by treating the samples and using both FAAS in a conventional way and a spectrophotometric method using diphenylcarbazide at the 95% confidence level (ANOVA test) [14].
• In Tris-washed thylakoids from vegetative cells, however, diphenylcarbazide can act as an electron donor to Photosystem II [15].

Analytical, diagnostic and therapeutic context of Diphenylcarbazide

• The potency of Aspergillus niger was evaluated in shake flask culture by absorption of chromium at pH 6 and temperature 30 degrees C. The results of the study indicated removal of more than 75% chromium by Aspergillus niger determined by diphenylcarbazide colorimetric assay and atomic absorption spectrophotometry after 7 days [16].

References