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Chemical Compound Review

Tillmans Reagent     4-(3,5-dichloro-4-hydroxy...

Synonyms: DCPIP, oxidized DCIP, oxidized DCPIP, CHEBI:945, CHEMBL198157, ...
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Disease relevance of Dichlorophenolindophenol


High impact information on Dichlorophenolindophenol


Chemical compound and disease context of Dichlorophenolindophenol


Biological context of Dichlorophenolindophenol


Anatomical context of Dichlorophenolindophenol


Associations of Dichlorophenolindophenol with other chemical compounds


Gene context of Dichlorophenolindophenol


Analytical, diagnostic and therapeutic context of Dichlorophenolindophenol

  • FdIIint is observed during reductive titrations with dithionite or hydrogen/hydrogenase or after partial oxidation of FdIIR by 2,6-dichlorophenolindophenol or air [32].
  • The reduced form of DCIP (DCIPH2) is measured amperometrically by flow-injection analysis [33].
  • For each enzyme, the specificity of cleavage was determined by analytical HPLC while the kinetic constants were obtained from a kinetic assay involving a cellobiose dehydrogenase purified from H. insolens as a coupled enzyme using 2,6-dichloroindophenol as the electron acceptor [34].
  • The core complex revealed electron-transfer activity (1,5-diphenylcarbazide----2,6-dichloroindophenol) of about 30 mumol reduced 2,6-dichloroindophenol/mg chlorophyll/h. The structural integrity was analyzed by electron microscopy [35].
  • The enzyme is therefore called "ecdysone oxidase". Two methods are described for the detection of ecdysone oxidase activity, one using a radiolabelled substrate which is separated from the product by thin-layer chromatography after the reaction, and the other using dichloroindophenol, which is discoloured by the redox reaction [36].


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