The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
Chemical Compound Review

NITROBLUE TETRAZOLIUM     2-[2-methoxy-4-[3-methoxy-4- [3-(4...

Synonyms: CPD-8129, CHEBI:7586, CHEMBL1185260, AC1L1SQA, AC1Q1ZEO, ...
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

High impact information on Nitrotetrazolium Blue

  • The NADPH oxidase inhibitor, diphenyleneiodonium, decreased NBT reduction to 9+/-1 pmol x min(-1) x mg(-1), whereas inhibitors of xanthine oxidase, mitochondrial oxidases, and nitric oxide synthase were ineffective [1].
  • The reduction of NBT in intact aortic rings was 30+/-2 pmol x min(-1) x mg(-1) and was significantly decreased by superoxide dismutase to 19+/-2 pmol x min(-1) x mg(-1) and by a synthetic superoxide dismutase mimic, Euk-8, to 11+/-2 pmol x min(-1) x mg(-1) [1].
  • Rats received 10 mg NBT via the abdominal aorta on day 3, 7, or 13 of pseudopregnancy and were autopsied 1 min later to prepare the histological sections [2].
  • On the other hand, simultaneous administration of 100 micrograms phorbol 12-myristate 13-acetate, which activates mononuclear phagocytes to produce superoxide radical, significantly increased the numbers of NBT-positive cells in the CL on day 7 of pseudopregnancy, but not in the CL on day 3 or 13 of pseudopregnancy [2].
  • The co-purification and the competitive inhibition of NOS by the NADPHd substrate, nitro blue tetrazolium (NBT), are proof that in insects the enzyme responsible for fixation-insensitive NADPHd activity is nitric oxide synthase [3].

Anatomical context of Nitrotetrazolium Blue

  • Oral administration of TJ-9 for 3 weeks did not affect the ability of these macrophages to produce superoxide anions evaluated in situ by liver perfusion with nitro blue tetrazolium (NBT) and phorbol myristate acetate (PMA) [4].

Associations of Nitrotetrazolium Blue with other chemical compounds


Gene context of Nitrotetrazolium Blue

  • After optimization of the permeabilization conditions, hGH mRNA could be visualized in each case studied when alkaline phosphatase/NBT-based detection was employed [5].


  1. Superoxide anion from the adventitia of the rat thoracic aorta inactivates nitric oxide. Wang, H.D., Pagano, P.J., Du, Y., Cayatte, A.J., Quinn, M.T., Brecher, P., Cohen, R.A. Circ. Res. (1998) [Pubmed]
  2. Progesterone inhibits superoxide radical production by mononuclear phagocytes in pseudopregnant rats. Sugino, N., Shimamura, K., Tamura, H., Ono, M., Nakamura, Y., Ogino, K., Kato, H. Endocrinology (1996) [Pubmed]
  3. Ca2+/calmodulin-dependent nitric oxide synthase in Apis mellifera and Drosophila melanogaster. Müller, U. Eur. J. Neurosci. (1994) [Pubmed]
  4. Regulation of hepatic macrophage function by oral administration of xiao-chai-hu-tang (sho-saiko-to, TJ-9) in rats. Fujiwara, K., Mochida, S., Nagoshi, S., Iijima, O., Matsuzaki, Y., Takeda, S., Aburada, M. Journal of ethnopharmacology. (1995) [Pubmed]
  5. Sensitive detection of human growth hormone mRNA in routinely formalin-fixed, paraffin-embedded transgenic mouse tissues by non-isotopic in situ hybridization. Ehrlein, J., Wanke, R., Weis, S., Brem, G., Hermanns, W. Histochemistry (1994) [Pubmed]
WikiGenes - Universities