Disease relevance of lon

• In addition, the native transposase is stabilized in lon- strains of Escherichia coli, and, in these protease-deficient strains, trans action of transposase is increased 10- to 100-fold [1].

High impact information on lon

• ATP hydrolysis is not stoichiometrically linked with proteolysis in the ATP-dependent protease La from Escherichia coli [2].
• Five different lon mutations, not related to the Mar phenotype, were also found in 12 of the 13 mutants [3].
• Role for tandem duplication and lon protease in AcrAB-TolC- dependent multiple antibiotic resistance (Mar) in an Escherichia coli mutant without mutations in marRAB or acrRAB [4].
• We have used Tn10 and lambda placMu mutagenesis to identify a chromosomal locus, slpA, that is required for alpA+ suppression of delta lon [5].
• We have previously found that plasmids carrying the Escherichia coli alp gene (now to be called alpA) suppress two phenotypes of a delta lon protease mutant, overproduction of capsular polysaccharide and sensitivity to UV light [5].

Chemical compound and disease context of lon

• A point mutant in the ATP-binding motif (GPPGVGK362T) of the ATP-dependent protease La from Escherichia coli was investigated in which the lysine at position 362 was replaced by an alanine [6].

Biological context of lon

• Suppression of these lon phenotypes is most likely explained by the increased degradation of the Lon substrates responsible for these phenotypes [5].
• Moreover, 2-D gel analysis revealed that the expression level of some scavenger proteins marks the lon mutant as being in a situation similar to oxidative stress [7].
• A point mutation within the ATP-binding site inactivates both catalytic functions of the ATP-dependent protease La (Lon) from Escherichia coli [6].

Other interactions of lon

• These results suggest that instability of the IS903 transposase is a major determinant of its cis action and that the La protease, product of the lon gene, is an important determinant of transposase instability [1].

Analytical, diagnostic and therapeutic context of lon

• Our RT-PCR analysis revealed an elevation of expression of the oxyS gene in the lon mutant [7].
• The results of Western blotting detected a decrease in the protein expression levels of BtuB and OmpF involved in colicin translocation in the lon mutant [7].

References