Disease relevance of phoR

• The pho-510 mutation is responsible for the phoR mutant constitutive phenotype and probably arose in E. coli K-12 58F+ after X-ray mutagenesis over 40 years ago [1].

High impact information on phoR

• B. subtilis phoR was placed in the Bacillus chromosome under the control of the Pspac promoter, which is IPTG inducible [2].
• The results suggest that phoB and phoR constitute a single operon whose promoter is located proximal to phoB [3].
• We constructed recombinant plasmids that contain a phoR'-'lacZ fusion gene to study the regulation of phoR expression [3].
• Regulation of the phosphate regulon of Escherichia coli: analysis of mutant phoB and phoR genes causing different phenotypes [4].
• Although an operon structure was suggested previously (K. Makino, H. Shinagawa, M. Amemura, and A. Nakata, J. Mol. Biol. 190:37-44 and 192:549-556, 1986), previous results showed only that phoR gene expression during phosphate limitation is dependent on the upstream phoB promoter [5].

Biological context of phoR

• Mutation in any one of the three regulator genes (phoR, T and S) is known to make the cell constitutive for APase synthesis and enable the mutants to form larger colonies on beta-glycerophosphate plate under the condition of excess inorganic phosphate [6].

Analytical, diagnostic and therapeutic context of phoR

• In Southern blot analysis with specific probes from very conserved regions of the phoR and phoB genes from Escherichia coli, a positive hybridization with S. acidocaldarius BC65 chromosomal DNA fragments was found [7].

References