The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
 
Gene Review

glgB  -  glycogen branching enzyme

Escherichia coli CFT073

 
 
Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.
 

Disease relevance of glgB

  • In order to increase the branching degree of potato tuber starch, the gene encoding branching enzyme (glgB) of Escherichia coli was expressed in the amylose-free potato mutant [1].
  • A Butyrivibrio fibrisolvens H17c glgB gene, was isolated by direct selection for colonies that produced clearing on starch azure plates [2].
  • The structural gene for the Bacillus stearothermophilus glycogen branching enzyme (glgB) was cloned in Escherichia coli [3].
  • The B. stearothermophilus glgB gene was preceded by a sequence with extensive similarity to promoters recognized by Bacillus subtilis RNA polymerase containing sigma factor H (E - sigma H) [3].
  • A single glgB gene Rv1326c is present in Mycobacterium tuberculosis [4].
 

High impact information on glgB

  • To enhance glgB expression, the first intron of rice starch-branching enzyme and the matrix attachment region (MAR) sequence from chicken lysozyme were included in the expression vector [5].
  • The glgB gene consisted of an open reading frame of 1,920 bp encoding a protein of 639 amino acids (calculated Mr, 73,875) with 46 to 50% sequence homology with other branching enzymes [2].
  • The nucleotide sequences of the Escherichia coli genome between the glycogen biosynthetic genes glgB and glgC, and 1170 bp of DNA which follows glgA have been determined [6].
  • The deduced amino acid (aa) sequence for the putative product shows significant similarity to the E. coli glycogen branching enzyme, and to several different glucan hydrolases and transferases [6].
  • The presence of active branching enzyme activity in crude sonicates of Rb. sphaeroides cells indicates that the genome contains a full-length glgB at another location [7].
 

Biological context of glgB

 

Associations of glgB with chemical compounds

  • Similar to SBEI, GBE branched amylopectin at a lower rate than did SBEII [8].
 

Analytical, diagnostic and therapeutic context of glgB

References

  1. Expression of Escherichia coli branching enzyme in tubers of amylose-free transgenic potato leads to an increased branching degree of the amylopectin. Kortstee, A.J., Vermeesch, A.M., de Vries, B.J., Jacobsen, E., Visser, R.G. Plant J. (1996) [Pubmed]
  2. Characterization of the Butyrivibrio fibrisolvens glgB gene, which encodes a glycogen-branching enzyme with starch-clearing activity. Rumbak, E., Rawlings, D.E., Lindsey, G.G., Woods, D.R. J. Bacteriol. (1991) [Pubmed]
  3. Molecular cloning and nucleotide sequence of the glycogen branching enzyme gene (glgB) from Bacillus stearothermophilus and expression in Escherichia coli and Bacillus subtilis. Kiel, J.A., Boels, J.M., Beldman, G., Venema, G. Mol. Gen. Genet. (1991) [Pubmed]
  4. Expression and characterization of alpha-(1,4)-glucan branching enzyme Rv1326c of Mycobacterium tuberculosis H37Rv. Garg, S.K., Alam, M.S., Kishan, K.V., Agrawal, P. Protein Expr. Purif. (2007) [Pubmed]
  5. Expression of Escherichia coli branching enzyme in caryopses of transgenic rice results in amylopectin with an increased degree of branching. Kim, W.S., Kim, J., Krishnan, H.B., Nahm, B.H. Planta (2005) [Pubmed]
  6. Analysis of the Escherichia coli glycogen gene cluster suggests that catabolic enzymes are encoded among the biosynthetic genes. Romeo, T., Kumar, A., Preiss, J. Gene (1988) [Pubmed]
  7. Cloning and sequencing of glycogen metabolism genes from Rhodobacter sphaeroides 2.4.1. Expression and characterization of recombinant ADP-glucose pyrophosphorylase. Igarashi, R.Y., Meyer, C.R. Arch. Biochem. Biophys. (2000) [Pubmed]
  8. Comparing the properties of Escherichia coli branching enzyme and maize branching enzyme. Guan, H., Li, P., Imparl-Radosevich, J., Preiss, J., Keeling, P. Arch. Biochem. Biophys. (1997) [Pubmed]
  9. Characterization and crystallization of an active N-terminally truncated form of the Escherichia coli glycogen branching enzyme. Hilden, I., Leggio, L.L., Larsen, S., Poulsen, P. Eur. J. Biochem. (2000) [Pubmed]
 
WikiGenes - Universities