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Gene Review:

ZWINT - ZW10 interacting kinetochore protein

Homo sapiens

Synonyms: HZwint-1, KNTC2AP, SIP30, ZW10 interactor, ZW10-interacting protein 1, ...

Starr, D.A. et al., Wang, H. et al., Obuse, C. et al., Musio, A. et al., Kops, G.J. et al.

Musio, Mariani, Montagna, Zambroni, Ascoli, Ried, Vezzoni,

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High impact information on ZWINT

Therefore, centromeric HP1 may be the base to anchor the hMis12 core complex that is enriched with coiled coils and extends to outer Zwint-1 during mitosis [1].
Zwint-1 is critical for recruiting ZW10 to unattached kinetochores [2].
Zwint-1 specifies the kinetochore association of ZW10 by interacting with its N-terminal domain [3].
These Zwint-1-suppressed cells display chromosome bridge phenotype with sister chromatids inter-connected [3].
Here we show that Zwint-1 is required and is sufficient for kinetochore localization of Zeste White 10 (ZW10) in HeLa cells [3].

Biological context of ZWINT

HZwint-1, a novel human kinetochore component that interacts with HZW10 [4].
HZwint-1 was also found to localize to neocentromeres and to the active centromere of dicentric chromosomes [4].
HZwint-1 thus appears to associate with all active centromeres, implying that it plays an important role in correct centromere function [4].
This localization pattern, combined with the two-hybrid results, suggests that HZwint-1 may play a role in targeting HZW10 to the kinetochore at prometaphase [4].
The kinetochore localization of HZwint-1 has been confirmed by following GFP fluorescence in HeLa cells transiently transfected with a plasmid encoding a GFP/HZwint-1 fusion protein [4].

Anatomical context of ZWINT

Finally, our studies show that Zwint-1 is a new component of the mitotic check-point, as cells lacking Zwint-1 fail to arrest in mitosis when exposed to microtubule inhibitors, yielding interphase cells with multinuclei [3].

Associations of ZWINT with chemical compounds

Immunofluorescence studies with sera raised against HZwint-1 protein revealed strong kinetochore staining in nocodazole-arrested chromosome spreads [4].

Physical interactions of ZWINT

HZwint-1 (Human ZW10 interacting protein-1) was identified in a yeast two hybrid screen for proteins that interact with HZW10 [4].

Other interactions of ZWINT

Nine polypeptides bind to human hMis12; two of these, HEC1 and Zwint-1, are authentic kinetochore proteins [1].
We concluded that INCENP, ZWINT-1, ZW10 gene inhibition results in cellular phenocopies of Roberts syndrome [5].
Human Zwint-1 specifies localization of Zeste White 10 to kinetochores and is essential for mitotic checkpoint signaling [3].

References

A conserved Mis12 centromere complex is linked to heterochromatic HP1 and outer kinetochore protein Zwint-1. Obuse, C., Iwasaki, O., Kiyomitsu, T., Goshima, G., Toyoda, Y., Yanagida, M. Nat. Cell Biol. (2004) [Pubmed]
ZW10 links mitotic checkpoint signaling to the structural kinetochore. Kops, G.J., Kim, Y., Weaver, B.A., Mao, Y., McLeod, I., Yates, J.R., Tagaya, M., Cleveland, D.W. J. Cell Biol. (2005) [Pubmed]
Human Zwint-1 specifies localization of Zeste White 10 to kinetochores and is essential for mitotic checkpoint signaling. Wang, H., Hu, X., Ding, X., Dou, Z., Yang, Z., Shaw, A.W., Teng, M., Cleveland, D.W., Goldberg, M.L., Niu, L., Yao, X. J. Biol. Chem. (2004) [Pubmed]
HZwint-1, a novel human kinetochore component that interacts with HZW10. Starr, D.A., Saffery, R., Li, Z., Simpson, A.E., Choo, K.H., Yen, T.J., Goldberg, M.L. J. Cell. Sci. (2000) [Pubmed]
Recapitulation of the Roberts syndrome cellular phenotype by inhibition of INCENP, ZWINT-1 and ZW10 genes. Musio, A., Mariani, T., Montagna, C., Zambroni, D., Ascoli, C., Ried, T., Vezzoni, P. Gene (2004) [Pubmed]

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