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Gene Review:

Cbr2 - carbonyl reductase 2

Mus musculus

Synonyms: AP27, Adipocyte protein P27, LCR, Lung carbonyl reductase, MLCR, ...

Nakanishi, M. et al., Wenz, H.M. et al., De Leo, A.A. et al., Gaudreault, C. et al., Rütters, H. et al., et al.

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Disease relevance of Cbr2

Proteins associated with lung tumor growth and/or metastasis, i.e., lung carbonyl reductase, differed in expression, as did tumor-associated expression of cell adhesion and membrane-bound proteins such as vinculin [1].

High impact information on Cbr2

Surprisingly, transient transfection of a beta-globin gene (epsilon, gamma, or beta) induces transcription of the LCR and intergenic regions from the chromosomal beta-globin locus in nonerythroid cell lines [2].
These results led us to propose that a threshold level of the clone 5 gene product (AP27 protein) is required for triggering adipocyte differentiation [3].
These results provide strong evidence that expression of AP27 is linked to adipogenic differentiation and that AP27 may be a component of an as-yet-uncharacterized signal-transduction pathway required for the triggering of adipocyte differentiation [3].
Furthermore, extinction of gamma gene expression in adult life is not completely autonomous and is incomplete when HS2 is the only LCR element present [4].
Mouse lung carbonyl reductase, a member of the short-chain dehydrogenase/reductase (SDR) family, exhibits coenzyme specificity for NADP(H) over NAD(H) [5].

Biological context of Cbr2

A database search using the predicted hamster P26h amino acid sequence revealed 85% identity with mouse AP27 protein and porcine carbonyl reductase, members of the short-chain dehydrogenase/reductase (SDR) family of proteins [6].
Analysis of the region 5' to the S. macroura epsilon (epsilon) globin gene revealed a region similar to the eutherian LCR, containing sequences and potential transcription factor binding sites with homology to eutherian hypersensitive sites 1 to 5 [7].

Associations of Cbr2 with chemical compounds

Switch of coenzyme specificity of mouse lung carbonyl reductase by substitution of threonine 38 with aspartic acid [5].
Using site-directed mutagenesis, we constructed a mutant mouse lung carbonyl reductase in which Thr-38 was replaced by Asp (T38D), and we compared kinetic properties of the mutant and wild-type enzymes in both forward and reverse reactions [5].
Lysine residues in the coenzyme-binding region of mouse lung carbonyl reductase [8].

Physical interactions of Cbr2

Crystallization of mouse lung carbonyl reductase complexed with NADPH and analysis of symmetry of its tetrameric molecule [9].

Other interactions of Cbr2

Within the olfactory epithelium, Cbr2 is a new anatomical marker for sustentacular cells [10].

References

Towards a lung adenocarcinoma proteome map: studies with SP-C/c-raf transgenic mice. Rütters, H., Zürbig, P., Halter, R., Borlak, J. Proteomics (2006) [Pubmed]
Intergenic transcription and transinduction of the human beta-globin locus. Ashe, H.L., Monks, J., Wijgerde, M., Fraser, P., Proudfoot, N.J. Genes Dev. (1997) [Pubmed]
Reduced expression of AP27 protein, the product of a growth factor-repressible gene, is associated with diminished adipocyte differentiation. Wenz, H.M., Hinck, L., Cannon, P., Navre, M., Ringold, G.M. Proc. Natl. Acad. Sci. U.S.A. (1992) [Pubmed]
Globin gene switching in transgenic mice carrying HS2-globin gene constructs. Roberts, N.A., Sloane-Stanley, J.A., Sharpe, J.A., Stanworth, S.J., Wood, W.G. Blood (1997) [Pubmed]
Switch of coenzyme specificity of mouse lung carbonyl reductase by substitution of threonine 38 with aspartic acid. Nakanishi, M., Matsuura, K., Kaibe, H., Tanaka, N., Nonaka, T., Mitsui, Y., Hara, A. J. Biol. Chem. (1997) [Pubmed]
Hamster sperm protein, p26h: a member of the short-chain dehydrogenase/reductase superfamily. Gaudreault, C., Lé garé, C., Bérubé, B., Sullivan, R. Biol. Reprod. (1999) [Pubmed]
Sequencing and mapping hemoglobin gene clusters in the Australian model dasyurid marsupial Sminthopsis macroura. De Leo, A.A., Wheeler, D., Lefevre, C., Cheng, J.F., Hope, R., Kuliwaba, J., Nicholas, K.R., Westerman, M., Graves, J.A. Cytogenet. Genome Res. (2005) [Pubmed]
Lysine residues in the coenzyme-binding region of mouse lung carbonyl reductase. Deyashiki, Y., Nakanishi, M., Sakai, M., Hara, A. Adv. Exp. Med. Biol. (1995) [Pubmed]
Crystallization of mouse lung carbonyl reductase complexed with NADPH and analysis of symmetry of its tetrameric molecule. Tanaka, N., Nonaka, T., Nakanishi, M., Deyashiki, Y., Hara, A. J. Biochem. (1995) [Pubmed]
Differentially expressed transcripts from phenotypically identified olfactory sensory neurons. Yu, T.T., McIntyre, J.C., Bose, S.C., Hardin, D., Owen, M.C., McClintock, T.S. J. Comp. Neurol. (2005) [Pubmed]

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LOC607806	Canis lupus familiaris
MGG_13518	Magnaporthe oryzae 70-15
NCU02097	Neurospora crassa OR74A
LOC688320	Rattus norvegicus
SPAC922.06	Schizosaccharomyces pombe 972h-

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