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Gene Review

Col4a2  -  collagen, type IV, alpha 2

Mus musculus

Synonyms: AU019502, Col4a-2, Collagen alpha-2(IV) chain
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Disease relevance of Col4a2

  • The recombinant mouse canstatin efficiently expressed in E. coli M15 after IPTG induction was monitored by SDS-PAGE and by Western blotting with an anti-hexahistidine tag antibody [1].
  • Nine copies of the hypoxia-response element were ligated upstream from the canstatin gene near the cytomegalovirus promoter [2].
  • Angiogenesis is crucial for the growth and metastasis of solid tumors with sizes larger than a few cubic millimeter Canstatin, the non-collagenous 1 (NC1) domain of alpha2 chain of type IV collagen, was previously shown to inhibit proliferation of endothelial cells in vitro and suppress in vivo tumor growth [3].
  • AIM: To examine the effect of canstatin, a newly discovered endogenous inhibitor of angiogenesis, in the treatment of pancreatic cancer in vivo [4].

High impact information on Col4a2


Anatomical context of Col4a2


Regulatory relationships of Col4a2


Analytical, diagnostic and therapeutic context of Col4a2

  • Canstatin protein expression was assessed by Western blot analysis in tumor tissues. pCMVCans and empty vector were used as controls in the in vivo assays [2].
  • We believe that the hypoxia-inducible canstatin-expressing vector is a promising gene therapy tool for antiangiogenesis research [2].
  • METHODS: The canstatin cDNA fragment was synthesized and amplified from the total RNA extracted from human placenta tissues by RT-PCR [4].
  • Twenty-four nude mice with orthotopic xenograft tumor were randomly divided into 3 groups 10 d after the inoculation, and were treated with PBS 0.3 mL, or canstatin 5 mg/kg, or 10 mg/kg per day for 3 wk intraperitoneally [4].


  1. Recombinant mouse canstatin inhibits chicken embryo chorioallantoic membrane angiogenesis and endothelial cell proliferation. Hou, W.H., Wang, T.Y., Yuan, B.M., Chai, Y.R., Jia, Y.L., Tian, F., Wang, J.M., Xue, L.X. Acta Biochim. Biophys. Sin. (Shanghai) (2004) [Pubmed]
  2. Enhancement of antiangiogenic effects of human canstatin with a hypoxia-regulated transgene vector in lung cancer model. Li, Y.Y., Qian, G.S., Huang, G.J., Chen, F., Qian, P., Yu, S.C., Wang, C.Z., Li, Q., Wang, J.C., Wu, G.M. Cancer journal (Sudbury, Mass.) (2006) [Pubmed]
  3. The C-terminal domain of canstatin suppresses in vivo tumor growth associated with proliferation of endothelial cells. He, G.A., Luo, J.X., Zhang, T.Y., Hu, Z.S., Wang, F.Y. Biochem. Biophys. Res. Commun. (2004) [Pubmed]
  4. Effects of recombinant human canstatin protein in the treatment of pancreatic cancer. He, X.P., Li, Z.S., Zhu, R.M., Tu, Z.X., Gao, J., Pan, X., Gong, Y.F., Jin, J., Man, X.H., Wu, H.Y., Xu, A.F. World J. Gastroenterol. (2006) [Pubmed]
  5. Canstatin, a novel matrix-derived inhibitor of angiogenesis and tumor growth. Kamphaus, G.D., Colorado, P.C., Panka, D.J., Hopfer, H., Ramchandran, R., Torre, A., Maeshima, Y., Mier, J.W., Sukhatme, V.P., Kalluri, R. J. Biol. Chem. (2000) [Pubmed]
  6. Homologs of genes and anonymous loci on human chromosome 13 map to mouse chromosomes 8 and 14. Koizumi, T., Hendel, E., Lalley, P.A., Tchetgen, M.B., Nadeau, J.H. Mamm. Genome (1995) [Pubmed]
  7. SRY-related HMG box 9 regulates the expression of Col4a2 through transactivating its enhancer element in mesangial cells. Sumi, E., Iehara, N., Akiyama, H., Matsubara, T., Mima, A., Kanamori, H., Fukatsu, A., Salant, D.J., Kita, T., Arai, H., Doi, T. Am. J. Pathol. (2007) [Pubmed]
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