Disease relevance of G1211R

• The putative DNA polymerase-encoding gene of African swine fever virus has been sequenced [1].
• In contrast to all other alpha-like DNA polymerases of DNA viruses, ASFV-specific DNA polymerase is resistant to aphidicolin [2].
• Previous comparisons of DNA polymerase sequences from vertebrate and invertebrate viruses suggested that ascoviruses are closely related to iridoviruses [3].
• Inhibition of ASFV replication by PAA suggests that this virus, like other herpesviruses, involves a virus-specific DNA polymerase in its replication mechanism [4].

High impact information on G1211R

• The African swine fever virus DNA polymerase X (ASFV Pol X or Pol X), the smallest known nucleotide polymerase, has recently been reported to be an extremely low fidelity polymerase that may be involved in strategic mutagenesis of the viral genome [5].
• Characterization of an African swine fever virus 20-kDa DNA polymerase involved in DNA repair [6].
• A DNA polymerase with specificity for five base pairs [7].
• The DNA polymerase gene of African swine fever virus (ASFV) was mapped by marker rescue experiments using a phosphonoacetic acid-resistant mutant and hybridization with an oligonucleotide probe designed from the most conserved motif of family B DNA polymerases [8].
• A unique feature of ASFV DNA polymerase is the presence of 13 tandem repeats of the sequence Ala-Gly-Asp-Pro near the carboxyl end of the molecule [8].

Biological context of G1211R

• DNA polymerase X (Pol X) from the African swine fever virus (ASFV) specifically binds intermediates in the single-nucleotide base-excision repair process, an activity indicative of repair function [9].
• Genetic identification and nucleotide sequence of the DNA polymerase gene of African swine fever virus [8].
• These results predict for pol X a substrate-induced conformational change triggered by the presence of DNA and the correct incoming nucleotide in the active site, as in DNA polymerase beta [10].
• Plasmid DNA was also replicated, which indicates that ASFV-specific DNA polymerase does not require a virus-specific origin of replication [11].
• Assuming a common cellular origin of viral DNA polymerase genes the corresponding amino acid sequences were chosen to construct a phylogenetic tree showing the relatedness among large DNA viruses of eucaryotes [12].

Anatomical context of G1211R

• The observed inhibition of the in vivo DNA synthesis could be related to the in vitro inhibition of a virus-induced DNA polymerase activity present in cytoplasmic extracts from infected VERO cells [13].

Associations of G1211R with chemical compounds

• The reaction was inhibited by phosphonoacetic acid, an inhibitor of ASFV-specific DNA polymerase [11].

Other interactions of G1211R

• We recently demonstrated that African swine fever virus DNA polymerase X (Pol X) is extremely error-prone during single-nucleotide gap-filling and that the downstream ASFV DNA ligase seals 3' mismatched nicks with high efficiency [14].

References