Disease relevance of NS1

• NS1, the major nonstructural parvovirus protein of the minute virus of mice, is a multifunctional protein responsible for several aspects of viral replication [1].
• The IHHNV putative NS-1 shares the highest aa sequence homology with the NS-1 of mosquito brevidensoviruses, Aedes densovirus and Aedes albopictus parvovirus [2].
• Minute virus of mice NS1 protein is a multifunctional phosphoprotein endowed with a variety of enzymatic and regulatory activities necessary for progeny virus particle production [3].

High impact information on NS1

• These are resolved by the viral initiator protein, NS1, but mechanisms employed at the two telomeres differ [4].
• These act as spacers between the NS1 and PIF binding sites, and their asymmetric distribution sets up active (OriL(TC)) and inactive (OriL(GAA)) forms of OriL [4].
• The minute virus of mice initiator protein, NS1, excises new copies of the left viral telomere in a single sequence orientation, dubbed flip, during resolution of the junction between monomer genomes in palindromic dimer intermediate duplexes [5].
• The parvovirus minute virus of mice NS1 protein is a multifunctional protein involved in a variety of processes during virus propagation, ranging from viral DNA replication to promoter regulation and cytotoxic action to the host cell [6].
• Replicative functions of minute virus of mice NS1 protein are regulated in vitro by phosphorylation through protein kinase C [7].

Biological context of NS1

• Levels of B19-DNA and B19-NS1 mRNA transcription increased in the presence of anti-B19 IgG antibodies, but this effect decreased in the presence of anti-Fc receptor antibodies, showing antibody-dependent enhancement by B19 infection [8].
• Analysis of NS1 clones derived from his longitudinal viral isolates showed the existence of quasi-species but genetic drift was not found [9].
• Two putative promoters, located upstream of the left and right ORFs, are presumed to regulate expression of NS and VP genes, respectively [2].
• When transfected to non-permissive cells, NS1 recruits the mitochondria cell death pathway [10].

Other interactions of NS1

• In this report, longitudinal genetic changes in NS1 and VP1 gene of B19 isolates from three AIDS patients with persistent B19 infection were studied [9].

Analytical, diagnostic and therapeutic context of NS1

• A new nested-polymerase chain reaction (nested-PCR) assay was developed to detect human parvovirus B19 DNA corresponding to the nonstructural protein in clinical specimens in a routine diagnostic laboratory [11].

References