Disease relevance of pol

• The viral genome has the coding capacity for the Moloney murine leukemia virus gag gene product and contains large deletions in pol and env genes [1].
• A mutant murine leukemia virus with a single missense codon in pol is defective in a function affecting integration [2].
• Linker-scanning libraries were generated within the 3' terminus of the Moloney murine leukemia virus (M-MuLV) pol gene encoding the connection-RNase H domains of reverse transcriptase (RT) as well as the structurally related M-MuLV and human immunodeficiency virus type 1 (HIV-1) integrase (IN) proteins [3].
• The cloned provirus was defective and contained a C-terminal end of the pol gene, a complete envelope gene, and a 3' long terminal repeat [4].
• We have cloned and expressed in Escherichia coli a section of the Moloney murine leukemia virus (Mo-MLV) pol gene which includes the entire coding region of mature reverse transcriptase (RT) plus 284 additional base pairs 3' to the coding region (Kotewicz et al., 1985) [5].

High impact information on pol

• The mutants could be divided into two classes depending on the phenotype and map position of the deletion within the pol gene [6].
• DNA sequence analysis of two SIRE-1 subclones revealed that SIRE-1 contains a long, uninterrupted, ORF between the 3' end of the pol ORF and the 3' long terminal repeat (LTR), a region that harbors the env gene in retroviral genomes [7].
• The F6A provirus is organized into 5'-LTR-gag-pol-env-LTR-3' regions; the gag and pol open reading frames are separated by an amber codon, and env is in a different reading frame [8].
• XC plaque assays of recombinant virus progeny on Fv-ln and Fv-lb cells indicated that the Fv-l host range was determined by sequences located between the BamHI site in the p30 region of the gag gene (1.6 kilobase pairs from the left end of the map) and the HindIII site located in the pol gene (2.9 kilobase pairs from the left end of the map) [9].
• Although neither pol alpha nor KF readily polymerized a natural dNTP opposite either 5- or 6-nitrobenzimidazole in the template strand, the enzymes did incorporate the analogues to generate novel base pairs [10].

Biological context of pol

• Functional analysis of reverse transcription by a frameshift pol mutant of murine leukemia virus [11].
• This new system was used to evaluate the contribution of drug-resistance mutations in the pol and env genes to overall viral replicative fitness (in the presence and absence of drug pressure) using direct growth competition experiments [12].

Analytical, diagnostic and therapeutic context of pol

• When we tested these mink cells by immune precipitation or by competition radioimmunoassay, we found that they synthesized gPr82env, but contained no detectable gag or pol proteins [13].

References