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Gene Review:

OXER1 - oxoeicosanoid (OXE) receptor 1

Homo sapiens

Synonyms: 5-oxo-ETE G-protein coupled receptor, G-protein coupled receptor 170, G-protein coupled receptor R527, G-protein coupled receptor TG1019, GPCR, ...

Hosoi, T. et al., Jones, C.E. et al., O'Flaherty, J.T. et al., Vrecl, M. et al.

Jones, Holden, Tenaillon, Bhatia, Seuwen, Tranter, Turner, Kettle, Bouhelal, Charlton, Nirmala, Jarai, Finan, O'Flaherty, Rogers, Paumi, Hantgan, Thomas, Clay, High, Chen, Willingham, Smitherman, Kute, Rao, Cramer, Morrow,

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High impact information on OXER1

We have conducted an in silico data base search for and cloned a novel G-protein-coupled receptor (GPCR) named TG1019 [1].
Dot and Northern blotting analyses showed that transcripts of the novel GPCR were expressed in various tissues except brain, and the expression was more intense in liver, kidney, peripheral leukocyte, lung, and spleen than in other tissues [1].
Conversely, polyunsaturated fatty acids such as docosahexaenoic acid and eicosapentaenoic acid showed antagonistic activities against TG1019 [1].
By GTP gamma S binding assay using the TG1019-G alpha(i1)-protein fusion expressed in insect cells, eicosanoids, and polyunsaturated fatty acids such as 5-oxo-6E,8Z,11Z,14Z-eicosatetraenoic acid (5-oxo-ETE), 5(S)-hydroperoxy-6E,8Z, 11Z,14Z-eicosatetraenoic acid, and arachidonic acid were identified to exhibit agonistic activities against TG1019 [1].
Quantitative reverse transcription-polymerase chain reaction analysis of the expression of GPCR R527 indicated a very high level of mRNA expression in eosinophils, with high expression also detected in neutrophils and lung macrophages [2].

Biological context of OXER1

Using a bioinformatics approach, we have isolated a novel G-protein-coupled receptor (GPCR), R527, and have demonstrated that this receptor shows no significant homology to previously deorphanized GPCRs [2].
These results are the first to implicate the OXE receptor in malignant cell growth and to show that 5-oxo-ETEs activate cell death programs as well as PPARgamma independently of this receptor [3].

Associations of OXER1 with chemical compounds

MDA-MB-231, MCF7, and SKOV3 cancer cells, but not HEK-293 cells, expressed mRNA for the leukocyte G protein-coupled 5-oxo-eicosatetraenoate (ETE) OXE receptor [3].

Other interactions of OXER1

The beta-arr2 R393E, R395E mutant also enhanced the signal window with other members of the GPCR family (neuropeptide Y type 2 receptor [NPY2-R] and TG1019 receptor) and was successfully applied in full-plate BRET(2)-based agonist and antagonist screening assays [4].

References

Identification of a novel human eicosanoid receptor coupled to G(i/o). Hosoi, T., Koguchi, Y., Sugikawa, E., Chikada, A., Ogawa, K., Tsuda, N., Suto, N., Tsunoda, S., Taniguchi, T., Ohnuki, T. J. Biol. Chem. (2002) [Pubmed]
Expression and characterization of a 5-oxo-6E,8Z,11Z,14Z-eicosatetraenoic acid receptor highly expressed on human eosinophils and neutrophils. Jones, C.E., Holden, S., Tenaillon, L., Bhatia, U., Seuwen, K., Tranter, P., Turner, J., Kettle, R., Bouhelal, R., Charlton, S., Nirmala, N.R., Jarai, G., Finan, P. Mol. Pharmacol. (2003) [Pubmed]
5-Oxo-ETE analogs and the proliferation of cancer cells. O'Flaherty, J.T., Rogers, L.C., Paumi, C.M., Hantgan, R.R., Thomas, L.R., Clay, C.E., High, K., Chen, Y.Q., Willingham, M.C., Smitherman, P.K., Kute, T.E., Rao, A., Cramer, S.D., Morrow, C.S. Biochim. Biophys. Acta (2005) [Pubmed]
Development of a BRET2 screening assay using beta-arrestin 2 mutants. Vrecl, M., Jorgensen, R., Pogacnik, A., Heding, A. Journal of biomolecular screening : the official journal of the Society for Biomolecular Screening. (2004) [Pubmed]

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