Disease relevance of Prkce

• Cell type-specific expression of the genes for the protein kinase C family: down regulation of mRNAs for PKC alpha and nPKC epsilon upon in vitro differentiation of a mouse neuroblastoma cell line neuro 2a [1].
• The gender-specific mechanisms of TCDD-induced toxicity appear to involve c-Src kinase, nPKC epsilon, and pan-Ras, as well as overlap in the cytosolic signal transduction pathways of TCDD and sex steroids [2].

High impact information on Prkce

• The implication of protein kinase C (PKC) isoforms cPKC-alpha, nPKC-epsilon, aPKC-lambda and aPKC-zeta in the transcriptional activation of a c-fos promoter-driven CAT-reporter construct by transforming Ha-Ras has been investigated [3].
• We conclude that under physiological conditions, conventional cPKC-alpha and novel nPKC-epsilon, but not atypical aPKC-lambda are responsible for MARCKS phosphorylation in intact NIH 3T3 fibroblasts [4].
• Northern blot analysis showed that the mRNA for nPKC eta is highly expressed in the lung and skin but, in contrast to other members of the PKC family, only slightly expressed in the brain. nPKC eta expressed in COS cells shows phorbol ester binding activity with a similar affinity to nPKC epsilon [5].
• As compared to vector control cell lines expressing only the hygromycin resistance gene, the nPKC epsilon overproducing cell lines exhibited a 7-13-fold increase in Ca(2+)-independent enzyme activity [6].
• We have overproduced the Ca(2+)-independent protein kinase C isoform, nPKC epsilon, in Rat 6 embryo fibroblasts, and examined the effects of this novel isoform on cell growth and transformation [6].

Anatomical context of Prkce

• Further, nPKC epsilon mRNA is expressed in some of these cell lines [1].

Other interactions of Prkce

• Modest increases in novel PKC-epsilon (nPKC-epsilon) expression and subcellular membrane partitioning were recorded during TMCH, but no changes were seen for PKC-delta or -eta [7].
• In a mouse neuroblast cell line, Neuro 2a, down modulation of mRNAs for both PKC alpha and nPKC epsilon was observed in association with in vitro differentiation [1].

References