Disease relevance of Tcte3

• The possible role of Tctex2 in t-complex transmission ratio distortion and sterility is discussed [1].
• Soon after the tetanus, twitch potentiation and increases of LC2 phosphorylation and of [Ca2+]i peak were similar in control conditions and in the presence of adrenaline [2].
• We have earlier demonstrated the presence in excess of a neutral protease that specifically cleaves LC2 in human hearts obtained at end stage idiopathic dilated cardiomyopathy (IDC) [3].

High impact information on Tcte3

• Combined with the recent identification of another putative t complex distorter, Tctex2, within the outer dynein arm, these results support the hypothesis that transmission ratio distortion (meiotic drive) of mouse t haplotypes involves dysfunction of both flagellar inner and outer dynein arms but does not require the cytoplasmic isozyme [4].
• Molecular analysis of a 19,000-Mr protein from the Chlamydomonas flagellum reveals that it is homologous to the t complex-encoded protein Tctex-2, which is a candidate for one of the distorter products that cause the extreme transmission ratio distortion (meiotic drive) of the murine t complex [5].
• These data raise the possibility that Tctex-2 is a sperm flagellar dynein component [5].
• Tctex1 and Tctex2 were originally described as potential distorters/sterility factors in the non-Mendelian transmission of t-haplotypes in mice [6].
• LC2, the chlamydomonas homologue of the t complex-encoded protein Tctex2, is essential for outer dynein arm assembly [7].

Biological context of Tcte3

• The Tctex1/Tctex2 class of dynein light chains. Dimerization, differential expression, and interaction with the LC8 protein family [8].
• The t-haplotype form of Tctex2 is aberrant in both the level of its expression and its primary amino acid sequence, but is nonetheless translated and transported to its normal location [1].
• Transformation of the deletion mutant with the cloned LC2/ODA12 gene restores the outer arms and rescues the motility phenotype [7].
• These results suggest that mouse Tctex2 might also be a light chain of flagellar outer arm dynein and that the abortive phosphorylation of Tctex2/outer arm dynein light chain might be related to the less progressive movement of sperm [9].
• Tctex2-related outer arm dynein light chain is phosphorylated at activation of sperm motility [9].

Anatomical context of Tcte3

• The characterization of Tctex2 (t-complex testis expressed 2) shows it to be one of several candidates for involvement in TRD [1].
• Tctex2: a sperm tail surface protein mapping to the t-complex [1].
• Variant cell lines (LC1, LC2) obtained by growth of mouse L cells (L) in cytostimulatory and cytotoxic doses, respectively, of rabbit anti-L cell antiserum AL) were found previously to be altered in many ways relative to the parent cell line [10].
• A neutral protease with an estimated Mr of about 26 kD and responsible for cleavage ofmyosin LC2 was isolated from hamster skeletal muscle [3].

Other interactions of Tcte3

• The Tctex1/Tctex2 family of dynein light chains associates with the intermediate chains at the base of the soluble dynein particle [8].

Analytical, diagnostic and therapeutic context of Tcte3

• Northern blot and in situ hybridization showed that Tctex4 is a novel gene that is expressed in mouse testis [11].

References