High impact information on Rorb

• The orphan nuclear receptor RORbeta is expressed in areas of the central nervous system which are involved in the processing of sensory information, including spinal cord, thalamus and sensory cerebellar cortices [1].
• However, in contrast to RAR and TR, the RZR beta AF-2 cannot function autonomously as a transactivation domain [2].
• Electrophoretic mobility shift assays reveal binding of RZR beta to monomeric response elements containing the sequence AnnTAGGTCA, but RZR beta-mediated transactivation of reporter genes is only achieved with two property spaced binding sites [2].
• We report that the orphan nuclear receptor RORbeta (retinoid-related orphan receptor beta) activates the S opsin gene (Opn1sw) through binding sites upstream of the gene [3].
• With-n-myc we propose a candidate gene regulated by ROR alpha/RZR beta and Nm23, based on the finding that the respective DNA binding sites in the first intron are conserved in several mammalian species [4].

Biological context of Rorb

• In neuronal cells, GaI-RZR beta fusion proteins function as potent transcriptional activators, whereas no transactivation can be observed in nonneuronal cells [2].
• The amino acid sequence of ROR gamma exhibits a 50 and 51% identity with those of ROR alpha/RZR alpha and RZR beta, respectively, whereas the DNA-binding domains were 89% identical [5].

Anatomical context of Rorb

• In addition, we discover that transcription factor Rorb is down-regulated in the barrelless mutant relative to control mice by quantitative comparison of expression patterns in layer IV somatosensory cortex [6].

Associations of Rorb with chemical compounds

• Mutational analyses demonstrate that the activation domain (AF-2) of RZR beta and RAR alpha are functionally interchangeable [2].

References