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Pcbp1  -  poly(rC) binding protein 1

Mus musculus

Synonyms: Alpha-CP1, Heterogeneous nuclear ribonucleoprotein E1, Poly(rC)-binding protein 1, WBP17, alphaCP-1, ...
 
 
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Disease relevance of Pcbp1

  • RESULTS: When compared to dams fed a folate-replete diet, those dams on a folate-depleted diet developed reduced red cell folates and hyperhomocysteinemia and an inverse dose-dependent upregulation of FR and hnRNP-E1 on GD17 without alterations in cell number in the majority of tissues [1].
 

High impact information on Pcbp1

  • Detection of this "alpha-complex" has been limited to in vitro studies, and the functional state of the alpha-globin mRNA targeted by alphaCP has not been defined [2].
  • PABP inhibited ErEN activity indirectly through an interaction with the alphaCP mRNA stability protein [3].
  • Sequestration of alphaCP resulted in an increase of ErEN cleavage activity, regardless of the polyadenylation state of the RNA [3].
  • Interestingly, the binding domain of one of the CCAAT factors, alpha-CP1, overlapped one alpha-IRP binding site. alpha-CP1 thus overlapped the binding domains of both alpha-CP2 and alpha-IRP [4].
  • In contrast, when sedimented in glycerol gradients containing 500 mM KCl, alpha-CP1 dissociated into at least two components [5].
 

Biological context of Pcbp1

 

Anatomical context of Pcbp1

  • This study examines the role of alphaCP in the decay of transcripts containing the collagen 3'-UTR in extracts obtained from NIH fibroblasts and quiescent and activated HSCs [8].
  • Fetuses from some groups were evaluated by specific biochemical, molecular, and immunohistochemical studies for FR, hnRNP-E1, and apoptosis [1].
 

Physical interactions of Pcbp1

  • Affinity purification of NF-M 3'-UTR-binding proteins from rat brain followed by mass spectrometry and immunoprecipitation assays identified heterogeneous nuclear ribonucleoprotein (hnRNP) K and hnRNP E1 as the proteins forming the 70- and 47-kDa complexes, respectively [9].
  • Targeted analysis of the polysomal RNP complexes revealed that alphaCP is specifically bound to actively translating alpha-globin mRNA [2].
 

Regulatory relationships of Pcbp1

 

Other interactions of Pcbp1

  • These data suggested that the alphaCP-1 gene was generated by retrotransposition of a fully processed alphaCP-2 mRNA and that this event occurred well before the mammalian radiation [6].
  • Two of the purified proteins, alpha-CP1 and alpha-CP2, have been shown previously to interact with distinct binding sites that overlap in the alpha-globin CCAAT box [5].
  • Since hnRNP E is known to stabilize mRNAs, these results raise the hypothesis that these proteins may contribute to the increases in cytoplasmic levels of NF-M mRNA that accompany axonal maturation [9].
 

Analytical, diagnostic and therapeutic context of Pcbp1

  • In addition chemical cross-linking, sedimentation, and gel filtration studies suggest that alpha-CP1 is a heterotrimeric factor composed of one polypeptide from each class [7].

References

  1. Maternal folate deficiency results in selective upregulation of folate receptors and heterogeneous nuclear ribonucleoprotein-E1 associated with multiple subtle aberrations in fetal tissues. Xiao, S., Hansen, D.K., Horsley, E.T., Tang, Y.S., Khan, R.A., Stabler, S.P., Jayaram, H.N., Antony, A.C. Birth defects research. Part A, Clinical and molecular teratology. (2005) [Pubmed]
  2. In vivo association of the stability control protein alphaCP with actively translating mRNAs. Ji, X., Kong, J., Liebhaber, S.A. Mol. Cell. Biol. (2003) [Pubmed]
  3. The poly(A)-binding protein and an mRNA stability protein jointly regulate an endoribonuclease activity. Wang, Z., Kiledjian, M. Mol. Cell. Biol. (2000) [Pubmed]
  4. Identification and characterization of multiple erythroid cell proteins that interact with the promoter of the murine alpha-globin gene. Barnhart, K.M., Kim, C.G., Banerji, S.S., Sheffery, M. Mol. Cell. Biol. (1988) [Pubmed]
  5. Purification of multiple erythroid cell proteins that bind the promoter of the alpha-globin gene. Kim, C.G., Barnhart, K.M., Sheffery, M. Mol. Cell. Biol. (1988) [Pubmed]
  6. A set of highly conserved RNA-binding proteins, alphaCP-1 and alphaCP-2, implicated in mRNA stabilization, are coexpressed from an intronless gene and its intron-containing paralog. Makeyev, A.V., Chkheidze, A.N., Liebhaber, S.A. J. Biol. Chem. (1999) [Pubmed]
  7. Physical characterization of the purified CCAAT transcription factor, alpha-CP1. Kim, C.G., Sheffery, M. J. Biol. Chem. (1990) [Pubmed]
  8. Regulation of alpha1(I) collagen messenger RNA decay by interactions with alphaCP at the 3'-untranslated region. Lindquist, J.N., Parsons, C.J., Stefanovic, B., Brenner, D.A. J. Biol. Chem. (2004) [Pubmed]
  9. Phylogenetically conserved binding of specific K homology domain proteins to the 3'-untranslated region of the vertebrate middle neurofilament mRNA. Thyagarajan, A., Szaro, B.G. J. Biol. Chem. (2004) [Pubmed]
 
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