High impact information on ESR1

• Between 2 and 3 ions were released when the progesterone receptor and the diluted estrogen receptor bound DNA [1].
• The suppression was reversed if cells were incubated with 10(-11) to 10(-7) M tamoxifen or ICI 182,780 combined with 10(-11) or 10(-9) M estradiol followed by incubation with 5 microg/ml insulin, indicating an estrogen receptor-mediated process [2].
• Expression results indicate the existence of AR and both ER subtypes in all 10 gastrointestinal compartments [3].
• A rapid two-step procedure was devised for the purification of the estrogen receptor from the calf uterus [4].
• A 900- to 1700-fold purification of the estrogen receptor was obtained using ammonium sulfate precipitation followed by dye affinity chromatography with Reactive Orange 14 immobilized to Sepharose [4].

Biological context of ESR1

• Subsequently, the estrogen receptor protein expression level in neutrophils obtained from cows in early lactation was compared with those from cows in late pregnancy [5].
• No difference was found for the estrogen receptor protein expression in neutrophils from cows in early lactation compared with late gestation neither were the endogenous 17beta-estradiol and progesterone levels correlated with the protein expression [5].
• When the dimerization constant of the progesterone receptor was measured, the value of approximately 7 nM obtained was 20-fold higher than the value of 0.3 nM reported for the estrogen receptor [1].
• We aimed to produce an estrogen-responsive reporter plasmid that would permit monitoring of estrogen receptor function in the uterus in vivo [6].

Anatomical context of ESR1

• Expression of the estrogen receptor in blood neutrophils of dairy cows during the periparturient period [5].
• Large antral follicles and early corpora lutea are the site, where both forms of estrogen receptor are expressed [7].

Associations of ESR1 with chemical compounds

• Increasing progesterone concentrations were associated with decreased PGR and ESR1 expression [8].
• The Reactive Orange 14-Sepharose was used to purify the estrogen receptor in the presence or absence of estradiol as well as to purify the progesterone receptor [4].
• Estrogen receptor purification by affinity chromatography using an orange triazine dye [4].
• The purified estrogen receptor retained its estradiol- and DNA-binding properties and sedimented into sucrose gradients as the 5 S receptor dimer [4].
• The affinity of zearalenone for the estrogen receptor was 2-3 times that of estradiol-17beta [9].

Other interactions of ESR1

• Western blot analysis confirmed ESR1 and PGR protein expression throughout the culture period [10].
• Further discrimination between the estrogen receptor subtypes alpha and beta revealed the expression of the estrogen receptor beta, whereas for the estrogen receptor alpha no reproducible positive signal could be obtained with the available antibodies [5].

Analytical, diagnostic and therapeutic context of ESR1

• A clear positive signal was obtained using flow cytometry for the estrogen receptor protein in bovine neutrophils [5].

References