The world's first wiki where authorship really matters (Nature Genetics, 2008). Due credit and reputation for authors. Imagine a global collaborative knowledge base for original thoughts. Search thousands of articles and collaborate with scientists around the globe.

wikigene or wiki gene protein drug chemical gene disease author authorship tracking collaborative publishing evolutionary knowledge reputation system wiki2.0 global collaboration genes proteins drugs chemicals diseases compound
Hoffmann, R. A wiki for the life sciences where authorship matters. Nature Genetics (2008)
Gene Review

NT5E  -  5'-nucleotidase, ecto (CD73)

Bos taurus

Welcome! If you are familiar with the subject of this article, you can contribute to this open access knowledge base by deleting incorrect information, restructuring or completely rewriting any text. Read more.

Disease relevance of NT5E


High impact information on NT5E


Anatomical context of NT5E


Associations of NT5E with chemical compounds


Other interactions of NT5E


Analytical, diagnostic and therapeutic context of NT5E

  • Bovine serum in NT or NTE buffers produced a thermostabilizing effect at 37 C not provided by the same serum concentration in complex cell culture media [3].
  • The liver lesion produced by the oral administration of chloroform is similar in the three species and is accompanied by the release of 5'-NT into the plasma of the sheep and calf but not of the horse [9].
  • Bovine liver ecto 5'-nucleotidase was solubilized by PIPLC, purified to a homogeneous state, and analyzed for the structures of GPI-anchor isoforms by HPLC and ESI-MS in combination with glycosidase treatments, after peptide-bond cleavage by CNBr or trypsin [10].
  • 0. High-performance liquid chromatography analysis reveals that the ecto-enzyme hydrolyzes epsilon-(ApnA) to give epsilon-adenosine-5'(n-1)-phosphate and epsilon-AMP, which are then further catabolized up to epsilon-adenosine via the membrane-bound nucleotidase system ecto-ATPase, ecto-ADPase (or apyrase), and ecto-5'-nucleotidase [11].


  1. Changes in ecto-5'-nucleotidase activity during early stages of vesicular stomatitis virus infection. Effects of concanavalin A. Daveze, J.L., Frappa, J., Beaudry, Y., Deloince, R., Fontanges, R. Acta Virol. (1983) [Pubmed]
  2. Biochemical and mass spectrometric characterization of soluble ecto-5'-nucleotidase from bull seminal plasma. Fini, C., Talamo, F., Cherri, S., Coli, M., Floridi, A., Ferrara, L., Scaloni, A. Biochem. J. (2003) [Pubmed]
  3. Thermal inactivation of rabies and other rhabdoviruses: stabilization by the chelating agent ethylenediaminetetraacetic acid at physiological temperatures. Michalski, F., Parks, N.F., Sokol, F., Clark, H.F. Infect. Immun. (1976) [Pubmed]
  4. Oviduct cells express the cyclic AMP-adenosine pathway. Cometti, B., Dubey, R.K., Imthurn, B., Jackson, E.K., Rosselli, M. Biol. Reprod. (2003) [Pubmed]
  5. A simple assay for ecto-5'-nucleotidase using intact pulmonary artery endothelial cells. Effect of endotoxin-induced cell injury. Bonitati, A.E., Agarwal, K.C., Rounds, S. Biochem. Pharmacol. (1993) [Pubmed]
  6. Presence of ectonucleotidases in cultured chromaffin cells: hydrolysis of extracellular adenine nucleotides. Torres, M., Pintor, J., Miras-Portugal, M.T. Arch. Biochem. Biophys. (1990) [Pubmed]
  7. Oxidative inactivation of brain ecto-5'-nucleotidase by thiols/Fe2+ system. Liu, X.W., Sok, D.E. Neurochem. Res. (2000) [Pubmed]
  8. Temperature effects on the structural and functional properties of GPI-anchored and anchor-less bull seminal plasma ecto-5'-nucleotidase. Fini, C., Coli, M., Floridi, A., D'Auria, S., Staiano, M., Nucci, R., Rossi, M. J. Biochem. (1998) [Pubmed]
  9. Further studies on the diagnostic value of gamma-glutamyl transpeptidase and 5'-nucleotidase in cattle, sheep and horses. Barakat, S.E., Ford, E.J. Res. Vet. Sci. (1988) [Pubmed]
  10. Studies on the GPI-anchored enzyme, hepatic 5'-nucleotidase. Microheterogeneity of the anchor, processing and localization. Ikezawa, H., Taguchi, R., Tamura, H., Kobayashi, T. Braz. J. Med. Biol. Res. (1994) [Pubmed]
  11. Ecto-enzymatic hydrolysis of diadenosine polyphosphates by cultured adrenomedullary vascular endothelial cells. Mateo, J., Miras-Portugal, M.T., Rotllán, P. Am. J. Physiol. (1997) [Pubmed]
WikiGenes - Universities