Disease relevance of NT5E

• Changes in ecto-5'-nucleotidase activity during early stages of vesicular stomatitis virus infection. Effects of concanavalin A [1].

High impact information on NT5E

• Biochemical and mass spectrometric characterization of soluble ecto-5'-nucleotidase from bull seminal plasma [2].
• Bovine serum protein components in complex cell culture media stabilized virus at 56 C, but at temperatures of less than or equal to 37 C, sodium tris (hydroxymethyl)-aminomethane (NT) buffer containing ethylenediaminetetraacetic acid (EDTA) (NTE) was a much more efficient stabilizer of virus infectivity [3].
• The extracellular cAMP-adenosine pathway refers to the local production of adenosine mediated by cAMP egress into the extracellular space, conversion of cAMP to AMP by ectophosphodiesterase (PDE), and the metabolism of AMP to adenosine by ecto-5'-nucleotidase [4].
• We hypothesized that endothelial cell injury alters ecto-5'-nucleotidase activity [5].
• A simple assay for ecto-5'-nucleotidase using intact pulmonary artery endothelial cells. Effect of endotoxin-induced cell injury [5].

Anatomical context of NT5E

• (Biochem J 250: 369-373, 1988) with rat adipocytes, we studied the properties of ecto-5'-nucleotidase in intact monolayers of cultured bovine pulmonary artery endothelial cells (BPAEC) and examined the effect of endotoxin on enzyme activity [5].
• The ecto 5'-nucleotidase activity of cultured chromaffin cells was more specific for the purine nucleotides, AMP and IMP, than for the pirimidine nucleotides, CMP and TMP [6].
• The phosphatidylinositol-specific phospholipase C released the ecto-5'-nucleotidase from the chromaffin cells in culture, thus suggesting an anchorage through phosphatidylinositol to plasma membranes [6].
• Study of ecto-5'-nucleotidase was performed in cultured epithelial cells, infected with vesicular stomatitis virus (VSV) [1].

Associations of NT5E with chemical compounds

• Ecto-5'-nucleotidase converts extracellular AMP to adenosine [5].
• Oxidative inactivation of brain ecto-5'-nucleotidase by thiols/Fe2+ system [7].

Other interactions of NT5E

• Temperature effects on the structural and functional properties of GPI-anchored and anchor-less bull seminal plasma ecto-5'-nucleotidase [8].

Analytical, diagnostic and therapeutic context of NT5E

• Bovine serum in NT or NTE buffers produced a thermostabilizing effect at 37 C not provided by the same serum concentration in complex cell culture media [3].
• The liver lesion produced by the oral administration of chloroform is similar in the three species and is accompanied by the release of 5'-NT into the plasma of the sheep and calf but not of the horse [9].
• Bovine liver ecto 5'-nucleotidase was solubilized by PIPLC, purified to a homogeneous state, and analyzed for the structures of GPI-anchor isoforms by HPLC and ESI-MS in combination with glycosidase treatments, after peptide-bond cleavage by CNBr or trypsin [10].
• 0. High-performance liquid chromatography analysis reveals that the ecto-enzyme hydrolyzes epsilon-(ApnA) to give epsilon-adenosine-5'(n-1)-phosphate and epsilon-AMP, which are then further catabolized up to epsilon-adenosine via the membrane-bound nucleotidase system ecto-ATPase, ecto-ADPase (or apyrase), and ecto-5'-nucleotidase [11].

References