Disease relevance of TSHB

• Treatment of tadpoles with goitrogenic compounds (sodium perchlorate and ethylenethiourea) increased TSHbeta mRNA expression (approximately twofold) and caused thyroid gland hyperplasia, confirming that feedback along the pituitary-thyroid axis was operative [1].

High impact information on TSHB

• In addition, the amino acid sequence region between residues bTSH beta 34-44 could be excised by trypsin digestion of bovine TSH beta (bTSH beta) without eliminating epitopic recognition by either MAb [2].
• Epinephrine (E) (10(-6) M) caused a significant increase in TSH, alpha-subunit, and TSH beta release into the medium (P less than 0.001, P less than 0.001, and P less than 0.01, respectively) [3].
• TRH and thyroid hormones affect the release of TSH and TSH beta to a greater extent than they do the alpha-subunit [4].
• Finally, total alpha and TSH beta subunit production was increased with TRH stimulation and decreased with thyroid hormone exposure [4].
• At the end of the treatment period, heifers were slaughtered and pituitary glands recovered for determination of gonadotrophin contents and levels of mRNA encoding FSH-beta, LH-beta, TSH-beta and common alpha glycoprotein hormone subunits using [32P]cDNA probes in total RNA dot and Northern blot assays [5].

Biological context of TSHB

• The resulting 891 basepairs (bp) of genomic DNA consisted of two coding exons of the canine TSH beta gene and an intron of 450 bp [6].
• The gene fragment that encodes mature TSH beta was cloned from the canine genomic DNA by direct polymerase chain reaction (PCR) using primers that were designed based on the consensus sequences from other species [6].

Anatomical context of TSHB

• Thus, immunoreactivity of TSH beta was detected in the secretory granules, the cytoplasmic matrix and in portions of the rough endoplasmic reticulum, in association with some membranes and inside some saccular structures [7].

Other interactions of TSHB

• Goat pituitary glands were immunohistochemically studied with antisera for bovine S-100 protein, rat LH beta, FSH, TSH beta, prolactin, ovine GH, and porcine ACTH1-39 by use of the superimposition technique on adjacent sections [8].

Analytical, diagnostic and therapeutic context of TSHB

• The specificity of immunoprecipitation was confirmed by competition with ovine alpha (but not with ovine LH beta or bovine TSH beta) [9].

References