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Gene Review:

SLC4A4 - solute carrier family 4 (sodium...

Bos taurus

Sun, X.C. et al., Diecke, F.P. et al., Ziegler, O. et al., Lane, J. et al.

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High impact information on SLC4A4

Investigations of corneal endothelium were made to resolve the apparent contradiction of the presence of sodium/bicarbonate cotransporter (NBC) in fresh and cultured cells and NBC's reported absence in isolated plasma membrane vesicles [1].
The finding of a novel chloride-activated NBC activity fully resolves the apparent contradiction between whole-cell and membrane vesicle preparations [1].
In corneal endothelium, there is evidence for basolateral entry of HCO(3)(-) into corneal endothelial cells via Na(+)-HCO(3)(-) cotransporter (NBC) proteins and for net HCO(3)(-) flux from the basolateral to the apical side [2].
Functional studies support the presence of the Na(+)-HCO(3)(-) cotransporter (NBC) in corneal endothelium and possibly corneal epithelium; however, molecular identification and membrane localization have not been reported [3].
Two isoforms of NBC have recently been cloned in kidney (kNBC) and pancreas (pNBC) [3].

Anatomical context of SLC4A4

Indirect immunofluorescence and confocal microscopy indicated that NBC locates predominantly to the basolateral membrane in corneal endothelial cells [3].
To test whether NBC is expressed in bovine cornea, Western blotting was performed, which showed a single band at approximately 130 kDa for freshly isolated and cultured endothelial cells, but no band for epithelium [3].
The binding of insulin to cultured IM-9 human lymphocytes was studied by flow cytometry using FITC-insulin and biotinylated insulins coupled to streptavidin-phycoerythrin (N alpha beta 1-biotinylinsulin (B-insulin) and N alpha B1-(biotinyl-epsilon-aminocaproyl)insulin (NBC-insulin)) [4].

Analytical, diagnostic and therapeutic context of SLC4A4

However, immunocytochemistry using optical sectioning by confocal microscopy and cryosections revealed the presence of NBC transporters in both the basolateral and apical cell membranes of cultured bovine corneal endothelial cells and freshly isolated rabbit endothelia [2].
These results suggest that B-insulin and NBC-insulin are suitable ligands for insulin binding studies using flow cytometry [4].

References

A chloride-activated Na(+)/HCO(3)(-)-coupled transport activity in corneal endothelial membranes. Lane, J., Wigham, C.G., Hodson, S.A. Biophys. J. (2000) [Pubmed]
Immunocytochemical localization of Na+-HCO3- cotransporters and carbonic anhydrase dependence of fluid transport in corneal endothelial cells. Diecke, F.P., Wen, Q., Sanchez, J.M., Kuang, K., Fischbarg, J. Am. J. Physiol., Cell Physiol. (2004) [Pubmed]
Expression and localization of Na(+)-HCO(3)(-) cotransporter in bovine corneal endothelium. Sun, X.C., Bonanno, J.A., Jelamskii, S., Xie, Q. Am. J. Physiol., Cell Physiol. (2000) [Pubmed]
Insulin binding to human cultured lymphocytes measured by flow cytometry using three ligands. Ziegler, O., Cantin, C., Germain, L., Dupuis, M., Sekaly, R.P., Drouin, P., Chiasson, J.L. Cytometry. (1994) [Pubmed]

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BOR4	Arabidopsis thaliana
BOR5	Arabidopsis thaliana
AGOS_AFR677C	Ashbya gossypii ATCC 10895
SLC4A4	Canis lupus familiaris
slc4a4a	Danio rerio
SLC4A4	Gallus gallus
SLC4A4	Homo sapiens
KLLA0C02123g	Kluyveromyces lactis NRRL Y-1140
SLC4A4	Macaca mulatta
MGG_15203	Magnaporthe oryzae 70-15
Slc4a4	Mus musculus
NCU01480	Neurospora crassa OR74A
SLC4A4	Pan troglodytes
Slc4a4	Rattus norvegicus
BOR1	Saccharomyces cerevisiae S288c
SPBC543.05c	Schizosaccharomyces pombe 972h-
slc4a4	Xenopus (Silurana) tropicalis

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